Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2281739..2282658 Replicon chromosome
Accession NZ_CP069067
Organism Mycobacterium tuberculosis strain N1268

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag JN146_RS10720 Protein ID WP_003900449.1
Coordinates 2282053..2282658 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag JN146_RS10715 Protein ID WP_003410124.1
Coordinates 2281739..2282044 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN146_RS10685 2276750..2278006 - 1257 WP_003902247.1 HNH endonuclease signature motif containing protein -
JN146_RS10690 2278360..2278935 + 576 WP_003410100.1 hypothetical protein -
JN146_RS10695 2278932..2279972 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
JN146_RS10700 2280214..2280933 + 720 WP_003410108.1 DUF433 domain-containing protein -
JN146_RS10705 2280923..2281339 + 417 WP_003410114.1 hypothetical protein -
JN146_RS10710 2281355..2281654 - 300 WP_003410120.1 hypothetical protein -
JN146_RS10715 2281739..2282044 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
JN146_RS10720 2282053..2282658 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN146_RS10725 2282683..2283042 - 360 WP_003410131.1 hypothetical protein -
JN146_RS10730 2283202..2283792 - 591 WP_057145698.1 hypothetical protein -
JN146_RS10735 2283831..2284067 - 237 WP_003410133.1 hypothetical protein -
JN146_RS10740 2284274..2285170 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T189193 WP_003900449.1 NZ_CP069067:c2282658-2282053 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T189193 NZ_CP090688:c425631-425314 [Burkholderia multivorans]
ATGCCTTACCTTGCTCCAACGTTCAGCATCCGGACGACCGCCGTGTTCGACGCATGGTTTGCCGGCCTGCAGGACCGCAC
CGCGAAGCGCCGCATTCAGGCGCGCATCGATCGGCTGTCGATGGGCAATCTGGGCAACTGGAAGTCCGTCGGGTCGCCCG
TCTACGAGATGCGCATCGATCACGGCCCCGGCTATCGCGTGTACTTCGTGCGGCGGCGCACGATATGGGTCGTCCTGCTG
TGCGGCGGCGACAAATCCACGCAGCGCGACGACATTCGCGCCGCCCATGCGATGCTCGAGCATCTCGACCTGGAGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT189193 WP_003410124.1 NZ_CP069067:c2282044-2281739 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT189193 NZ_CP090688:c425310-425011 [Burkholderia multivorans]
ATGAGCAAGATCAGCACGCGGCCGTGGGATTCGGCCGAACACCTGAAGACCGACGACGACATGGCCGAGTATTTCGAAGC
CTGCCTCCAGGAAGGCGGCGACGATCCGGCATTCATCGCGCACGCGCTCGGCGTGATCGCGCGCGCCCGCGGCATGTCGC
AAGTCGCGCGCGATGCGGGGCTATCGCGCGAGGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References