Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3533155..3533825 Replicon chromosome
Accession NZ_CP069066
Organism Mycobacterium tuberculosis strain N1201

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag JN147_RS16635 Protein ID WP_003899954.1
Coordinates 3533155..3533499 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag JN147_RS16640 Protein ID WP_003899955.1
Coordinates 3533496..3533825 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN147_RS16600 3528228..3529088 + 861 WP_003416628.1 alpha/beta hydrolase -
JN147_RS16605 3529063..3529578 + 516 WP_187704705.1 nitroreductase family deazaflavin-dependent oxidoreductase -
JN147_RS16610 3529594..3529805 + 212 Protein_3283 (R)-hydratase -
JN147_RS16615 3529818..3530111 + 294 WP_003416635.1 hypothetical protein -
JN147_RS16620 3530399..3531688 + 1290 WP_003416640.1 ATP-binding protein -
JN147_RS16625 3532035..3532469 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
JN147_RS16630 3532472..3532924 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN147_RS16635 3533155..3533499 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN147_RS16640 3533496..3533825 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
JN147_RS16645 3534363..3534710 + 348 WP_003899956.1 DUF2384 domain-containing protein -
JN147_RS16650 3534707..3535327 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
JN147_RS16655 3535487..3536752 - 1266 WP_003909837.1 hypothetical protein -
JN147_RS16660 3536920..3537129 + 210 WP_003416778.1 hypothetical protein -
JN147_RS16665 3537376..3538410 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T189150 WP_003899954.1 NZ_CP069066:3533155-3533499 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T189150 NZ_CP090673:c3282375-3281974 [Burkholderia multivorans]
ATGCCGCGCTACATGCTCGACACCAACATGTGCATCTATCTGATGAAGAACCAGCCGGAGCAGGTCGCGCGGCGTTTCGC
GCAGTGCTACACGGGGGACGTCGTCATGTCGGCGATCACCTATGCGGAACTCGAGTACGGCGTGACGCTATGCACCAATC
CTGCCCGCGAACGCCGCAATCTCGCCGCGCTGATCGAGGATATCCCGGTGGCGCCGTTCGACGTTGCGGCTGCGCAGGCA
TACGGCCCCATTCGAGAGGCGACGCGCGAACGCAAGAAGGATCATCTCGACAAGCTGATCGGCGCGCACGCCGTATCGCT
CGACGTCGTGCTGGTGACCAACAACGAGCGTGATTTCGCGAGTTATCCGGGCTTGAGGCTGGAGAACTGGCTGATCGGCT
AG

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT189150 WP_003899955.1 NZ_CP069066:3533496-3533825 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT189150 NZ_CP090673:c3282611-3282375 [Burkholderia multivorans]
ATGCATACCACCCGGGTCTTCCGGAATGGCAACTCGCAGGCGGTGCGGATTCCTGCGGATCTTGCTTATGAGCGCAGCGA
TATCGAACTCGAAATCGAGCGTGTCGGTGACGAAATTCGCATCCGGCCTGCGCGGCGGCCGCTGACCGGCGTGCTCGGCA
AGTTCGCGAAATTCGGGCCCGATTTCATGGCCGAGGGGCGCGGCGATCAGGAACAGGCCGAGCGCGAGGGTTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References