Detailed information of TA system TA189075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2250887..2251806
• Replicon: chromosome
• Accession: NZ_CP069065
• Organism: Mycobacterium tuberculosis strain N0091

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: L7N4R2
• Locus tag: JN148_RS10570
• Protein ID: WP_003900449.1
• Coordinates: 2251201..2251806 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: JN148_RS10565
• Protein ID: WP_003410124.1
• Coordinates: 2250887..2251192 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN148_RS10535	2245898..2247154	-	1257	WP_003902247.1	HNH endonuclease signature motif containing protein	-
JN148_RS10540	2247508..2248083	+	576	WP_003410100.1	hypothetical protein	-
JN148_RS10545	2248080..2249120	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
JN148_RS10550	2249362..2250081	+	720	WP_003410108.1	DUF433 domain-containing protein	-
JN148_RS10555	2250071..2250487	+	417	WP_003910489.1	hypothetical protein	-
JN148_RS10560	2250503..2250802	-	300	WP_003410120.1	hypothetical protein	-
JN148_RS10565	2250887..2251192	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
JN148_RS10570	2251201..2251806	-	606	WP_003900449.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JN148_RS10575	2251831..2252190	-	360	WP_003410131.1	hypothetical protein	-
JN148_RS10580	2252350..2252940	-	591	WP_057145698.1	hypothetical protein	-
JN148_RS10585	2252979..2253215	-	237	WP_003410133.1	hypothetical protein	-
JN148_RS10590	2253422..2254318	-	897	WP_003410136.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22744.96 Da
• Isoelectric Point: 7.3073

>T189075 WP_003900449.1 NZ_CP069065:c2251806-2251201 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T189075 NZ_CP090632:c2278432-2278016 [Burkholderia contaminans]
GTGACCACGCTCTACATGCTCGATACGAACATCTGTTCGTTCATCATGCGCGAGCGGCCGCCCGTGGTGCTGTCCCGGTT
GCAATCGTGCGTCAATGCGCAACACCGGATCGTCGTCTCGGCAGTTAGCTATGCCGAAATGCGCTTCGGCGCGGTCGGCG
GGAAGGCATCGCCGAAGCATGCGGAACTCGTGACGGCCTTTGTTTCGCGACTGGATGGCGTGCTGTCGTGGGACACGGCG
GCCGTCGACGCAACGGCCGCGATTCGTGCGGATTTCGCCTCGCGCGGCACGCCGATCGGCACCAACGACGCATCGATCGC
GGGCCATGCGATTGCGGCCGGTGCGGTGCTCGTCACCCACGACAGCCGCGAGTTCGGCCGTGTCGCCGGGTTGACGCTCG
AAGACTGGGCGGCCTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT189075 WP_003410124.1 NZ_CP069065:c2251192-2250887 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT189075 NZ_CP090632:c2278695-2278429 [Burkholderia contaminans]
ATGCGCACCGTTTCCATTTTCAAGAACGCCCGTAACCAGGCAATCCGCATCCCGAAGGACATGGAGTTCGAGGGGGTGAC
CGAACTCGAGATTCGCCGCGAGGGCGACACACTGCTGCTGCTGCCGGTGCGGCCCACATGGCTCTCGTTCGCGAGCGAAC
CGCCGGCCGACGCCGACTTCCTTGCCGAACGCCCGTCCGTCATCGAGCCCGGCCGCTTCGACCTGTCCGACGGTGGCGGC
GCCGCACCGACGGAGTCCGGCCAGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BV20

Antitoxin

• AlphaFold DB: A0A7U4BV30