Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3536640..3537310 Replicon chromosome
Accession NZ_CP069064
Organism Mycobacterium tuberculosis strain N1202

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag JN149_RS16645 Protein ID WP_003899954.1
Coordinates 3536640..3536984 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag JN149_RS16650 Protein ID WP_003899955.1
Coordinates 3536981..3537310 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN149_RS16610 3531713..3532573 + 861 WP_003416628.1 alpha/beta hydrolase -
JN149_RS16615 3532548..3533063 + 516 WP_187704705.1 nitroreductase family deazaflavin-dependent oxidoreductase -
JN149_RS16620 3533079..3533290 + 212 Protein_3285 (R)-hydratase -
JN149_RS16625 3533303..3533596 + 294 WP_003416635.1 hypothetical protein -
JN149_RS16630 3533884..3535173 + 1290 WP_003416640.1 ATP-binding protein -
JN149_RS16635 3535520..3535954 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
JN149_RS16640 3535957..3536409 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN149_RS16645 3536640..3536984 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN149_RS16650 3536981..3537310 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
JN149_RS16655 3537848..3538195 + 348 WP_003899956.1 DUF2384 domain-containing protein -
JN149_RS16660 3538192..3538812 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
JN149_RS16665 3538972..3540237 - 1266 WP_003909837.1 hypothetical protein -
JN149_RS16670 3540405..3540614 + 210 WP_003416778.1 hypothetical protein -
JN149_RS16675 3540861..3541895 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T189034 WP_003899954.1 NZ_CP069064:3536640-3536984 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T189034 NZ_CP090622:c4636771-4636238 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT189034 WP_003899955.1 NZ_CP069064:3536981-3537310 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT189034 NZ_CP090622:c4637048-4636782 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References