Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2259110..2260029 Replicon chromosome
Accession NZ_CP069064
Organism Mycobacterium tuberculosis strain N1202

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag JN149_RS10565 Protein ID WP_003900449.1
Coordinates 2259424..2260029 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag JN149_RS10560 Protein ID WP_003410124.1
Coordinates 2259110..2259415 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN149_RS10530 2254121..2255377 - 1257 WP_003902247.1 HNH endonuclease signature motif containing protein -
JN149_RS10535 2255731..2256306 + 576 WP_003410100.1 hypothetical protein -
JN149_RS10540 2256303..2257343 + 1041 WP_031666895.1 ImmA/IrrE family metallo-endopeptidase -
JN149_RS10545 2257585..2258304 + 720 WP_003410108.1 DUF433 domain-containing protein -
JN149_RS10550 2258294..2258710 + 417 WP_003910489.1 hypothetical protein -
JN149_RS10555 2258726..2259025 - 300 WP_003410120.1 hypothetical protein -
JN149_RS10560 2259110..2259415 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
JN149_RS10565 2259424..2260029 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN149_RS10570 2260054..2260413 - 360 WP_003410131.1 hypothetical protein -
JN149_RS10575 2260573..2261163 - 591 WP_057145698.1 hypothetical protein -
JN149_RS10580 2261202..2261438 - 237 WP_003410133.1 hypothetical protein -
JN149_RS10585 2261645..2262541 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T189017 WP_003900449.1 NZ_CP069064:c2260029-2259424 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T189017 NZ_CP090611:c4226278-4225967 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT189017 WP_003410124.1 NZ_CP069064:c2259415-2259110 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT189017 NZ_CP090611:c4225966-4225676 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References