Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 5077465..5078067 Replicon chromosome
Accession NZ_CP068830
Organism Escherichia coli strain RIVM_C018707

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag JNN82_RS24485 Protein ID WP_000897305.1
Coordinates 5077756..5078067 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JNN82_RS24480 Protein ID WP_000356395.1
Coordinates 5077465..5077755 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN82_RS24445 5073089..5073991 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
JNN82_RS24450 5073988..5074623 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
JNN82_RS24455 5074620..5075549 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
JNN82_RS24460 5075731..5075973 - 243 WP_001309881.1 ribbon-helix-helix domain-containing protein -
JNN82_RS24465 5076192..5076410 - 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
JNN82_RS24470 5076829..5077107 - 279 WP_001315112.1 hypothetical protein -
JNN82_RS24475 5077159..5077380 - 222 WP_001550354.1 hypothetical protein -
JNN82_RS24480 5077465..5077755 - 291 WP_000356395.1 helix-turn-helix domain-containing protein Antitoxin
JNN82_RS24485 5077756..5078067 - 312 WP_000897305.1 hypothetical protein Toxin
JNN82_RS24490 5078296..5079204 + 909 WP_001550353.1 alpha/beta hydrolase -
JNN82_RS24495 5079372..5081186 - 1815 WP_001550352.1 hypothetical protein -
JNN82_RS24500 5081602..5082543 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
JNN82_RS24505 5082588..5083025 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T188787 WP_000897305.1 NZ_CP068830:c5078067-5077756 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T188787 NZ_CP090561:483462-483596 [Xanthomonas citri pv. malvacearum]
ATGAAGCGTGCAATTGTGCTGCTGGTGTTATCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT188787 WP_000356395.1 NZ_CP068830:c5077755-5077465 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT188787 NZ_CP090561:483235-483387 [Xanthomonas citri pv. malvacearum]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGTAGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGTGCGGGCAACAAGGTCGAGAAAACGTCCGAAAAATGCAGCGACGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References