Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4079765..4080459 Replicon chromosome
Accession NZ_CP068830
Organism Escherichia coli strain RIVM_C018707

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag JNN82_RS19660 Protein ID WP_001263491.1
Coordinates 4079765..4080163 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag JNN82_RS19665 Protein ID WP_000554755.1
Coordinates 4080166..4080459 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN82_RS19630 4074934..4076178 - 1245 WP_000189541.1 esterase FrsA -
- 4075594..4075674 - 81 NuclAT_11 - -
- 4075594..4075674 - 81 NuclAT_11 - -
- 4075594..4075674 - 81 NuclAT_11 - -
- 4075594..4075674 - 81 NuclAT_11 - -
JNN82_RS19635 4076270..4076728 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
JNN82_RS19640 4076989..4078446 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
JNN82_RS19645 4078503..4078855 - 353 Protein_3847 peptide chain release factor H -
JNN82_RS19650 4078851..4079057 - 207 Protein_3848 RtcB family protein -
JNN82_RS19655 4079303..4079755 - 453 WP_019842500.1 GNAT family N-acetyltransferase -
JNN82_RS19660 4079765..4080163 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JNN82_RS19665 4080166..4080459 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JNN82_RS19670 4080511..4081566 - 1056 WP_001550655.1 DNA polymerase IV -
JNN82_RS19675 4081637..4082422 - 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
JNN82_RS19680 4082394..4084106 + 1713 Protein_3854 flagellar biosynthesis protein FlhA -
JNN82_RS19685 4084211..4084489 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JNN82_RS19690 4084482..4084838 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4069701..4080459 10758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T188780 WP_001263491.1 NZ_CP068830:c4080163-4079765 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T188780 NZ_CP068830:c4080163-4079765 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT188780 WP_000554755.1 NZ_CP068830:c4080459-4080166 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT188780 NZ_CP068830:c4080459-4080166 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References