Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2062658..2063160 Replicon chromosome
Accession NZ_CP068830
Organism Escherichia coli strain RIVM_C018707

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JNN82_RS09855 Protein ID WP_000767829.1
Coordinates 2062906..2063160 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JNN82_RS09850 Protein ID WP_001259255.1
Coordinates 2062658..2062909 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN82_RS09825 2057836..2058903 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JNN82_RS09830 2058903..2059973 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
JNN82_RS09835 2059970..2061274 - 1305 WP_039267630.1 histidinol dehydrogenase -
JNN82_RS09840 2061280..2062179 - 900 WP_000131789.1 ATP phosphoribosyltransferase -
JNN82_RS09845 2062325..2062375 - 51 WP_071593552.1 his operon leader peptide -
JNN82_RS09850 2062658..2062909 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JNN82_RS09855 2062906..2063160 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JNN82_RS09860 2063243..2064067 + 825 WP_000754737.1 SDR family oxidoreductase -
JNN82_RS09865 2064113..2065042 + 930 WP_000803351.1 LysR family transcriptional regulator -
JNN82_RS09870 2065257..2065319 + 63 WP_010723108.1 membrane protein YoeI -
JNN82_RS09875 2065309..2066667 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JNN82_RS09880 2066846..2067904 + 1059 WP_023568741.1 transport protein YeeE -
JNN82_RS09885 2067918..2068145 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T188764 WP_000767829.1 NZ_CP068830:2062906-2063160 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T188764 NZ_CP090547:2256693-2256998 [Xylella fastidiosa subsp. sandyi]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCCGTCGCTCGTGCTCGTAATTTAA
GTCAGCTGGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT188764 WP_001259255.1 NZ_CP068830:2062658-2062909 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT188764 NZ_CP090547:2256393-2256689 [Xylella fastidiosa subsp. sandyi]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATATCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References