Detailed information of TA system TA188763

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1297289..1298016
• Replicon: chromosome
• Accession: NZ_CP068830
• Organism: Escherichia coli strain RIVM_C018707

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: JNN82_RS06305
• Protein ID: WP_000547555.1
• Coordinates: 1297289..1297600 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN82_RS06310
• Protein ID: WP_000126294.1
• Coordinates: 1297597..1298016 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN82_RS06275	1292781..1293542	+	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
JNN82_RS06280	1293536..1294162	+	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
JNN82_RS06285	1294302..1295441	+	1140	WP_001272592.1	murein hydrolase activator NlpD	-
JNN82_RS06290	1295504..1296109	+	606	Protein_1236	sigma-70 family RNA polymerase sigma factor	-
JNN82_RS06295	1296172..1296876	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
JNN82_RS06300	1296928..1297122	+	195	Protein_1238	hydrogenase maturation peptidase HycI	-
JNN82_RS06305	1297289..1297600	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
JNN82_RS06310	1297597..1298016	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
JNN82_RS06315	1298095..1299519	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
JNN82_RS06320	1299528..1300985	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
JNN82_RS06325	1301245..1302255	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
JNN82_RS06330	1302404..1302931	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1296172..1296876	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T188763 WP_000547555.1 NZ_CP068830:1297289-1297600 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T188763 NZ_CP090547:2256393-2256689 [Xylella fastidiosa subsp. sandyi]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATATCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT188763 WP_000126294.1 NZ_CP068830:1297597-1298016 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT188763 NZ_CP090547:2256693-2256998 [Xylella fastidiosa subsp. sandyi]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCCGTCGCTCGTGCTCGTAATTTAA
GTCAGCTGGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Structures

Toxin

• AlphaFold DB: V0YLE2