Detailed information of TA system TA188757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5006125..5006727
• Replicon: chromosome
• Accession: NZ_CP068829
• Organism: Escherichia coli strain RIVM_C030453

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN50_RS24045
• Protein ID: WP_000897305.1
• Coordinates: 5006416..5006727 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN50_RS24040
• Protein ID: WP_000356395.1
• Coordinates: 5006125..5006415 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN50_RS24005	5001749..5002651	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN50_RS24010	5002648..5003283	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN50_RS24015	5003280..5004209	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN50_RS24020	5004391..5004633	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN50_RS24025	5004852..5005070	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN50_RS24030	5005489..5005767	-	279	WP_001315112.1	hypothetical protein	-
JNN50_RS24035	5005819..5006040	-	222	WP_001550354.1	hypothetical protein	-
JNN50_RS24040	5006125..5006415	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN50_RS24045	5006416..5006727	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN50_RS24050	5006956..5007864	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN50_RS24055	5008032..5009846	-	1815	WP_001550352.1	hypothetical protein	-
JNN50_RS24060	5010262..5011203	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN50_RS24065	5011248..5011685	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T188757 WP_000897305.1 NZ_CP068829:c5006727-5006416 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188757 NZ_CP090547:c1523590-1523285 [Xylella fastidiosa subsp. sandyi]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCCGTCGCTCGTGCTCGTAATTTAA
GTCAGCTGGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT188757 WP_000356395.1 NZ_CP068829:c5006415-5006125 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188757 NZ_CP090547:c1523890-1523594 [Xylella fastidiosa subsp. sandyi]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATATCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7