Detailed information of TA system TA188751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4007991..4008685
• Replicon: chromosome
• Accession: NZ_CP068829
• Organism: Escherichia coli strain RIVM_C030453

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: JNN50_RS19245
• Protein ID: WP_001263491.1
• Coordinates: 4007991..4008389 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: JNN50_RS19250
• Protein ID: WP_000554755.1
• Coordinates: 4008392..4008685 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN50_RS19215	4003160..4004404	-	1245	WP_000189541.1	esterase FrsA	-
-	4003820..4003900	-	81	NuclAT_10	-	-
-	4003820..4003900	-	81	NuclAT_10	-	-
-	4003820..4003900	-	81	NuclAT_10	-	-
-	4003820..4003900	-	81	NuclAT_10	-	-
JNN50_RS19220	4004496..4004954	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JNN50_RS19225	4005215..4006672	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JNN50_RS19230	4006729..4007081	-	353	Protein_3767	peptide chain release factor H	-
JNN50_RS19235	4007077..4007283	-	207	Protein_3768	RtcB family protein	-
JNN50_RS19240	4007529..4007981	-	453	WP_019842500.1	GNAT family N-acetyltransferase	-
JNN50_RS19245	4007991..4008389	-	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN50_RS19250	4008392..4008685	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN50_RS19255	4008737..4009792	-	1056	WP_001550655.1	DNA polymerase IV	-
JNN50_RS19260	4009863..4010648	-	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
JNN50_RS19265	4010620..4012332	+	1713	Protein_3774	flagellar biosynthesis protein FlhA	-
JNN50_RS19270	4012437..4012715	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JNN50_RS19275	4012708..4013064	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3997927..4008685	10758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T188751 WP_001263491.1 NZ_CP068829:c4008389-4007991 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188751 NZ_CP090547:461953-462234 [Xylella fastidiosa subsp. sandyi]
ATGCGGAGGATTAGTCAGACTGGCCAGTTCAAGCGCGACTACAAGCGCGAAGCTAAGGGCCAGCATCGCGCCACGCTTGA
TGAGGAACTAATACACGTCGTAGAAGCCTTGGCCTGTGATCATCCGTTGGAGCCACGCCACCACGATCACGCACTAACTG
GCGACTGGAAAGATCACCGTGATTGCCACATCAAACCCGACTTGGTACTGATATACCGGAAGCCCGACAACGAGACATTG
CAGCTCGTGCGGATTGGCTCACACAGCGAACTAGGCTTGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT188751 WP_000554755.1 NZ_CP068829:c4008685-4008392 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188751 NZ_CP090547:461703-461966 [Xylella fastidiosa subsp. sandyi]
ATGTCTGCAAACGCAGTTGTCCGTGCTCGCGTTGACGCGCACATCAAAGAAGAAGCATCAACCGTACTGGCGACGATGGG
CCTAAGCGTGTCTGACGCGTTCCGTATGATGCTGACCCGCATTGCCCGCGAGAAGGCGCTGCCGTTCGAGCCGCTGGTGC
CGAACGCAACCACTATCGCAGCCATGCGCGAAGCCCGCGCTGGTCACCTCAAGAGCTTTAACAGCATTGAGGGGCTCATG
GCGGACTTGCATGCGGAGGATTAG

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5