188706

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 731135..731862 Replicon chromosome
Accession NZ_CP068828
Organism Escherichia coli strain RIVM_C030443

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag JNN35_RS03580 Protein ID WP_000550189.1
Coordinates 731135..731449 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag JNN35_RS03585 Protein ID WP_000560253.1
Coordinates 731446..731862 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN35_RS03560 727301..728287 - 987 WP_001377074.1 Gfo/Idh/MocA family oxidoreductase -
JNN35_RS03565 728366..729049 - 684 WP_001183054.1 vancomycin high temperature exclusion protein -
JNN35_RS03570 729126..729629 - 504 WP_001309773.1 M48 family metallopeptidase -
JNN35_RS03575 729714..730850 + 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
JNN35_RS03580 731135..731449 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
JNN35_RS03585 731446..731862 + 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JNN35_RS03590 731907..733925 - 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
JNN35_RS03595 734096..736447 - 2352 WP_000695439.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T188706 WP_000550189.1 NZ_CP068828:731135-731449 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T188706 NZ_CP090539:951178-951591 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT188706 WP_000560253.1 NZ_CP068828:731446-731862 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT188706 NZ_CP090539:950931-951197 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References