Detailed information of TA system TA188697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4115492..4116186
• Replicon: chromosome
• Accession: NZ_CP068827
• Organism: Escherichia coli strain RIVM_C030371

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: JNN58_RS19650
• Protein ID: WP_001263491.1
• Coordinates: 4115788..4116186 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: JNN58_RS19645
• Protein ID: WP_000554755.1
• Coordinates: 4115492..4115785 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN58_RS19620	4111113..4111469	-	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
JNN58_RS19625	4111462..4111740	-	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JNN58_RS19630	4111845..4113557	-	1713	Protein_3831	flagellar biosynthesis protein FlhA	-
JNN58_RS19635	4113529..4114314	+	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
JNN58_RS19640	4114385..4115440	+	1056	WP_001550655.1	DNA polymerase IV	-
JNN58_RS19645	4115492..4115785	+	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN58_RS19650	4115788..4116186	+	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN58_RS19655	4116196..4116648	+	453	WP_019842500.1	GNAT family N-acetyltransferase	-
JNN58_RS19660	4116894..4117100	+	207	Protein_3837	RtcB family protein	-
JNN58_RS19665	4117096..4117448	+	353	Protein_3838	peptide chain release factor H	-
JNN58_RS19670	4117505..4118962	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JNN58_RS19675	4119223..4119681	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JNN58_RS19680	4119773..4121017	+	1245	WP_000189541.1	esterase FrsA	-
-	4120277..4120357	+	81	NuclAT_11	-	-
-	4120277..4120357	+	81	NuclAT_11	-	-
-	4120277..4120357	+	81	NuclAT_11	-	-
-	4120277..4120357	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T188697 WP_001263491.1 NZ_CP068827:4115788-4116186 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188697 NZ_CP090535:c4228529-4228245 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT188697 WP_000554755.1 NZ_CP068827:4115492-4115785 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188697 NZ_CP090535:c4228761-4228519 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5