Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4115492..4116186 Replicon chromosome
Accession NZ_CP068827
Organism Escherichia coli strain RIVM_C030371

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag JNN58_RS19650 Protein ID WP_001263491.1
Coordinates 4115788..4116186 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag JNN58_RS19645 Protein ID WP_000554755.1
Coordinates 4115492..4115785 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN58_RS19620 4111113..4111469 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
JNN58_RS19625 4111462..4111740 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JNN58_RS19630 4111845..4113557 - 1713 Protein_3831 flagellar biosynthesis protein FlhA -
JNN58_RS19635 4113529..4114314 + 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
JNN58_RS19640 4114385..4115440 + 1056 WP_001550655.1 DNA polymerase IV -
JNN58_RS19645 4115492..4115785 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JNN58_RS19650 4115788..4116186 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JNN58_RS19655 4116196..4116648 + 453 WP_019842500.1 GNAT family N-acetyltransferase -
JNN58_RS19660 4116894..4117100 + 207 Protein_3837 RtcB family protein -
JNN58_RS19665 4117096..4117448 + 353 Protein_3838 peptide chain release factor H -
JNN58_RS19670 4117505..4118962 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
JNN58_RS19675 4119223..4119681 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
JNN58_RS19680 4119773..4121017 + 1245 WP_000189541.1 esterase FrsA -
- 4120277..4120357 + 81 NuclAT_11 - -
- 4120277..4120357 + 81 NuclAT_11 - -
- 4120277..4120357 + 81 NuclAT_11 - -
- 4120277..4120357 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T188697 WP_001263491.1 NZ_CP068827:4115788-4116186 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T188697 NZ_CP090535:c4228529-4228245 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT188697 WP_000554755.1 NZ_CP068827:4115492-4115785 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT188697 NZ_CP090535:c4228761-4228519 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References