Detailed information of TA system TA188690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3136086..3136688
• Replicon: chromosome
• Accession: NZ_CP068827
• Organism: Escherichia coli strain RIVM_C030371

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN58_RS14975
• Protein ID: WP_000897305.1
• Coordinates: 3136086..3136397 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN58_RS14980
• Protein ID: WP_000356395.1
• Coordinates: 3136398..3136688 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN58_RS14955	3131128..3131565	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JNN58_RS14960	3131610..3132551	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN58_RS14965	3132967..3134781	+	1815	WP_001550352.1	hypothetical protein	-
JNN58_RS14970	3134949..3135857	-	909	WP_001550353.1	alpha/beta hydrolase	-
JNN58_RS14975	3136086..3136397	+	312	WP_000897305.1	hypothetical protein	Toxin
JNN58_RS14980	3136398..3136688	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN58_RS14985	3136773..3136994	+	222	WP_001550354.1	hypothetical protein	-
JNN58_RS14990	3137046..3137324	+	279	WP_001315112.1	hypothetical protein	-
JNN58_RS14995	3137743..3137961	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN58_RS15000	3138180..3138422	+	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN58_RS15005	3138604..3139533	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN58_RS15010	3139530..3140165	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN58_RS15015	3140162..3141064	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T188690 WP_000897305.1 NZ_CP068827:3136086-3136397 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188690 NZ_CP090535:c1047255-1046857 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT188690 WP_000356395.1 NZ_CP068827:3136398-3136688 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188690 NZ_CP090535:c1047482-1047255 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7