Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2203094..2203893 Replicon chromosome
Accession NZ_CP068827
Organism Escherichia coli strain RIVM_C030371

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag JNN58_RS10490 Protein ID WP_000347270.1
Coordinates 2203429..2203893 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag JNN58_RS10485 Protein ID WP_001551693.1
Coordinates 2203094..2203429 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN58_RS10470 2198879..2199649 - 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -
JNN58_RS10475 2199665..2200999 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
JNN58_RS10480 2201374..2202945 + 1572 WP_033878411.1 galactarate dehydratase -
JNN58_RS10485 2203094..2203429 + 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JNN58_RS10490 2203429..2203893 + 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JNN58_RS10495 2203948..2204757 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JNN58_RS10500 2205006..2206286 + 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
JNN58_RS10505 2206309..2206782 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JNN58_RS10510 2206793..2207572 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JNN58_RS10515 2207562..2208440 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JNN58_RS10520 2208458..2208892 + 435 WP_000948837.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2194332..2203893 9561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T188687 WP_000347270.1 NZ_CP068827:2203429-2203893 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T188687 NZ_CP090535:318063-318431 [Salmonella enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT188687 WP_001551693.1 NZ_CP068827:2203094-2203429 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT188687 NZ_CP090535:317845-318066 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References