Detailed information of TA system TA188666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5053012..5053614
• Replicon: chromosome
• Accession: NZ_CP068826
• Organism: Escherichia coli strain RIVM_C030300

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN53_RS24275
• Protein ID: WP_000897305.1
• Coordinates: 5053303..5053614 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN53_RS24270
• Protein ID: WP_000356395.1
• Coordinates: 5053012..5053302 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN53_RS24235	5048636..5049538	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN53_RS24240	5049535..5050170	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN53_RS24245	5050167..5051096	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN53_RS24250	5051278..5051520	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN53_RS24255	5051739..5051957	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN53_RS24260	5052376..5052654	-	279	WP_001315112.1	hypothetical protein	-
JNN53_RS24265	5052706..5052927	-	222	WP_001550354.1	hypothetical protein	-
JNN53_RS24270	5053012..5053302	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN53_RS24275	5053303..5053614	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN53_RS24280	5053843..5054751	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN53_RS24285	5054919..5056733	-	1815	WP_001550352.1	hypothetical protein	-
JNN53_RS24290	5057149..5058090	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN53_RS24295	5058135..5058572	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T188666 WP_000897305.1 NZ_CP068826:c5053614-5053303 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188666 NZ_CP090529:c4780558-4780247 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT188666 WP_000356395.1 NZ_CP068826:c5053302-5053012 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188666 NZ_CP090529:c4780246-4779956 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7