Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4042458..4043152 Replicon chromosome
Accession NZ_CP068826
Organism Escherichia coli strain RIVM_C030300

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag JNN53_RS19470 Protein ID WP_001263491.1
Coordinates 4042458..4042856 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag JNN53_RS19475 Protein ID WP_000554755.1
Coordinates 4042859..4043152 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN53_RS19440 4037627..4038871 - 1245 WP_000189541.1 esterase FrsA -
- 4038287..4038367 - 81 NuclAT_10 - -
- 4038287..4038367 - 81 NuclAT_10 - -
- 4038287..4038367 - 81 NuclAT_10 - -
- 4038287..4038367 - 81 NuclAT_10 - -
JNN53_RS19445 4038963..4039421 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
JNN53_RS19450 4039682..4041139 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
JNN53_RS19455 4041196..4041548 - 353 Protein_3812 peptide chain release factor H -
JNN53_RS19460 4041544..4041750 - 207 Protein_3813 RtcB family protein -
JNN53_RS19465 4041996..4042448 - 453 WP_019842500.1 GNAT family N-acetyltransferase -
JNN53_RS19470 4042458..4042856 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JNN53_RS19475 4042859..4043152 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JNN53_RS19480 4043204..4044259 - 1056 WP_001550655.1 DNA polymerase IV -
JNN53_RS19485 4044330..4045115 - 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
JNN53_RS19490 4045087..4046799 + 1713 Protein_3819 flagellar biosynthesis protein FlhA -
JNN53_RS19495 4046904..4047182 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JNN53_RS19500 4047175..4047531 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4032394..4043152 10758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T188659 WP_001263491.1 NZ_CP068826:c4042856-4042458 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T188659 NZ_CP090529:2144657-2144760 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT188659 WP_000554755.1 NZ_CP068826:c4043152-4042859 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT188659 NZ_CP090529:c2144762-2144617 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References