Detailed information of TA system TA188644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1221162..1221889
• Replicon: chromosome
• Accession: NZ_CP068826
• Organism: Escherichia coli strain RIVM_C030300

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: JNN53_RS05885
• Protein ID: WP_000547555.1
• Coordinates: 1221162..1221473 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN53_RS05890
• Protein ID: WP_000126294.1
• Coordinates: 1221470..1221889 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN53_RS05860	1217097..1218806	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
JNN53_RS05865	1218816..1219358	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
JNN53_RS05870	1219358..1220125	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
JNN53_RS05875	1220122..1220532	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
JNN53_RS05880	1220525..1220995	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
JNN53_RS05885	1221162..1221473	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
JNN53_RS05890	1221470..1221889	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
JNN53_RS05895	1221968..1223392	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
JNN53_RS05900	1223401..1224858	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
JNN53_RS05905	1225118..1226128	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
JNN53_RS05910	1226277..1226804	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T188644 WP_000547555.1 NZ_CP068826:1221162-1221473 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T188644 NZ_CP090522:c1334039-1333770 [Streptococcus parasuis]
ATGGCTTATAAACTTGTTCTCAGCGATGATGCTTTGAAGCAGTTGAAAAAAATGGACAGGCATGTAGGGATGATGCTTGC
TAAGGATTTGAAAAAAAGATTGGACGGTTTGGAAAATCCAAGACAGTTTGGCAAGGCCTTGGTAGGAGACTATAAGGGCC
TGTGGCGGTATAGAGTGGGGAATTATCGTGTAATTTGCGATATTATTGATAACAAAATGGTCATCTTAGCTCTTGAAATG
GGGCATCGGAAAGACATTTATCGGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT188644 WP_000126294.1 NZ_CP068826:1221470-1221889 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT188644 NZ_CP090522:c1334256-1334029 [Streptococcus parasuis]
ATGGGAATTGTTTCTTTGCGATTAAATGAAACAGAAGAAGAAATTTTCAGAAGCTATGCCATTCATACTGGAAAAAGCCT
GTCTGAGTTATTCAAATCAGCACTTATTGAACAGATAGAAGACCAGTTGGACTATGAAATTGGTATCAAGGCTTTGAAGA
AATTCCAAGAAAATCCTGTTACACACTCGATTGATGATGTGCTTGCGGAGTTAGAAGATGGCTTATAA

Structures

Toxin

• AlphaFold DB: V0YLE2