Detailed information of TA system TA188636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4220329..4221128
• Replicon: chromosome
• Accession: NZ_CP068825
• Organism: Escherichia coli strain RIVM_C030256

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: B7NDB6
• Locus tag: JNN79_RS20255
• Protein ID: WP_000347269.1
• Coordinates: 4220664..4221128 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0YUB5
• Locus tag: JNN79_RS20250
• Protein ID: WP_001309780.1
• Coordinates: 4220329..4220664 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN79_RS20235	4216114..4216884	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
JNN79_RS20240	4216900..4218234	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
JNN79_RS20245	4218609..4220180	+	1572	WP_001273738.1	galactarate dehydratase	-
JNN79_RS20250	4220329..4220664	+	336	WP_001309780.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JNN79_RS20255	4220664..4221128	+	465	WP_000347269.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JNN79_RS20260	4221183..4221992	-	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
JNN79_RS20265	4222241..4223521	+	1281	WP_000681943.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JNN79_RS20270	4223544..4224017	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JNN79_RS20275	4224028..4224807	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
JNN79_RS20280	4224797..4225675	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JNN79_RS20285	4225693..4226127	+	435	WP_000948818.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4211087..4221128	10041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T188636 WP_000347269.1 NZ_CP068825:4220664-4221128 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Nucleotide

• Length: 465 bp

>T188636 NZ_CP090518:1396796-1397101 [Xylella fastidiosa subsp. fastidiosa]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAGCTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12345.94 Da
• Isoelectric Point: 4.8616

>AT188636 WP_001309780.1 NZ_CP068825:4220329-4220664 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT188636 NZ_CP090518:1396496-1396792 [Xylella fastidiosa subsp. fastidiosa]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATCTCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG

Structures

Toxin

• AlphaFold DB: A0A829IY86

Antitoxin

• AlphaFold DB: V0YUB5