Detailed information of TA system TA188605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5053078..5053680
• Replicon: chromosome
• Accession: NZ_CP068824
• Organism: Escherichia coli strain RIVM_C030197

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN63_RS24265
• Protein ID: WP_000897305.1
• Coordinates: 5053369..5053680 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN63_RS24260
• Protein ID: WP_000356395.1
• Coordinates: 5053078..5053368 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN63_RS24225	5048702..5049604	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN63_RS24230	5049601..5050236	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN63_RS24235	5050233..5051162	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN63_RS24240	5051344..5051586	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN63_RS24245	5051805..5052023	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN63_RS24250	5052442..5052720	-	279	WP_001315112.1	hypothetical protein	-
JNN63_RS24255	5052772..5052993	-	222	WP_001550354.1	hypothetical protein	-
JNN63_RS24260	5053078..5053368	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN63_RS24265	5053369..5053680	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN63_RS24270	5053909..5054817	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN63_RS24275	5054985..5056799	-	1815	WP_001550352.1	hypothetical protein	-
JNN63_RS24280	5057215..5058156	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN63_RS24285	5058201..5058638	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T188605 WP_000897305.1 NZ_CP068824:c5053680-5053369 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188605 NZ_CP090511:c2599520-2599092 [Xylella fastidiosa subsp. multiplex]
ATGATTGTCTTGGATACCAATGTTGTTTCCGAAGCAATGAAACCCGAGCCGGATGCAGCAGTCCGAACCTGGCTCAACGA
GCAGATGAGCGTCACGTTGTACCTATCCAGTGTGACGTTATCTGAACTGTTGTTTGGAATCGCAGTGTTGCCCACAAGTA
AGCGCAAGGACATGTTGGCTCGTACCCTTGATGGCTTGCTTGATCTGTTCAATGAACGGGTGTTGCCGTTCGATACGGAC
GCGGCACGCCACTATGCTGAACTGGCAGTGAAAGCGAGAAACAATGGGCGCGGCTTTCCCACACCGGATGGCTATATCGC
GGCAATTGCTGCATCACGAGGTTACATAGTGGCTTCCCGCGACACCTCAGCCTATGAGTCAAGTGGTGTGCAAGTCATCA
ATCCGTGGCAATACAGCAAACAAACATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT188605 WP_000356395.1 NZ_CP068824:c5053368-5053078 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188605 NZ_CP090511:c2599775-2599521 [Xylella fastidiosa subsp. multiplex]
ATGGCCATGTTGACAGTGCGTAATTTGCCTGATGAAGTCCATCGCGCCTTGCGGGTTCGAGCCGCCACACACGGCCGCAG
TACAGAAGCGGAAGTACGTGAGATTTTAACGAACATAGTCAAGCCGGATGAGCGTGTTCGCATTGGCGATGCCTTGGCTA
TCCTGGGACGCGAGATCGGACTAAATAACGAGGATTTCACGACCTTTGATAAGGTGCGAGACAAGACGCAGGCCGAGCCG
ATGAGATTTGAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7