Detailed information of TA system TA188598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4042503..4043197
• Replicon: chromosome
• Accession: NZ_CP068824
• Organism: Escherichia coli strain RIVM_C030197

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: JNN63_RS19460
• Protein ID: WP_001263491.1
• Coordinates: 4042503..4042901 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: JNN63_RS19465
• Protein ID: WP_000554755.1
• Coordinates: 4042904..4043197 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN63_RS19430	4037672..4038916	-	1245	WP_000189541.1	esterase FrsA	-
-	4038332..4038412	-	81	NuclAT_10	-	-
-	4038332..4038412	-	81	NuclAT_10	-	-
-	4038332..4038412	-	81	NuclAT_10	-	-
-	4038332..4038412	-	81	NuclAT_10	-	-
JNN63_RS19435	4039008..4039466	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JNN63_RS19440	4039727..4041184	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JNN63_RS19445	4041241..4041593	-	353	Protein_3810	peptide chain release factor H	-
JNN63_RS19450	4041589..4041795	-	207	Protein_3811	RtcB family protein	-
JNN63_RS19455	4042041..4042493	-	453	WP_019842500.1	GNAT family N-acetyltransferase	-
JNN63_RS19460	4042503..4042901	-	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN63_RS19465	4042904..4043197	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN63_RS19470	4043249..4044304	-	1056	WP_001550655.1	DNA polymerase IV	-
JNN63_RS19475	4044375..4045160	-	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
JNN63_RS19480	4045132..4046844	+	1713	Protein_3817	flagellar biosynthesis protein FlhA	-
JNN63_RS19485	4046949..4047227	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JNN63_RS19490	4047220..4047576	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4032439..4043197	10758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T188598 WP_001263491.1 NZ_CP068824:c4042901-4042503 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188598 NZ_CP090511:1423635-1423901 [Xylella fastidiosa subsp. multiplex]
TTGGCCTGGACGATTGATTACACCGACACGGCCAAGCAGCAGTTACGCAAGCTCGACAAGCACATGGCACGGCGCATTGT
CGATTTCATGGATGAGCGCATCGCCGGACTAGAAAATCCACGCAGCAGTGGTAAAGCATTAACCGGACCACTTGGTGGCT
TTTGGCGTTATCGTGTTGGTGATTTTCGGGTCGTCTGCGACATTCAAGATAGCGTTTTGCGCGTCCTTGTAGTGCGTGTT
GGTCACCGGGGCGAGATTTACCGATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT188598 WP_000554755.1 NZ_CP068824:c4043197-4042904 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188598 NZ_CP090511:1423424-1423651 [Xylella fastidiosa subsp. multiplex]
ATGGCGACTTCGATTCGCCTCTCTCCTGAAATGGAGCAAAGGCTTAACTCTCTTGCCTCCCATACGGGACGCACAAAGGC
TTACTACCTGCGTGAAATCATTGAGCATGGCATTGAGGAAATGGAGGATTACTACCTTGCTGCCGATGTGTTGGAGCGTG
TCCGTCATGGACAAGAACAGGTGCATTCTGCTGCCGACGTGAGGAAAACGCTTGGCCTGGACGATTGA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5