Detailed information of TA system TA188583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1221167..1221894
• Replicon: chromosome
• Accession: NZ_CP068824
• Organism: Escherichia coli strain RIVM_C030197

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: JNN63_RS05885
• Protein ID: WP_000547555.1
• Coordinates: 1221167..1221478 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN63_RS05890
• Protein ID: WP_000126294.1
• Coordinates: 1221475..1221894 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN63_RS05860	1217102..1218811	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
JNN63_RS05865	1218821..1219363	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
JNN63_RS05870	1219363..1220130	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
JNN63_RS05875	1220127..1220537	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
JNN63_RS05880	1220530..1221000	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
JNN63_RS05885	1221167..1221478	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
JNN63_RS05890	1221475..1221894	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
JNN63_RS05895	1221973..1223397	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
JNN63_RS05900	1223406..1224863	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
JNN63_RS05905	1225123..1226133	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
JNN63_RS05910	1226282..1226809	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T188583 WP_000547555.1 NZ_CP068824:1221167-1221478 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T188583 NZ_CP090508:c2590108-2589842 [Xylella fastidiosa subsp. morus]
TTGGCCTGGACGATTGATTACACCGACACGGCCAAGCAGCAGTTACGCAAGCTCGACAAGCACATGGCGCGGCGCATTGT
CGATTTCATGGATGAGCGCATCGCCGGACTAGAAAACCCACGCAGCAGTGGTAAAGCATTAACCGGACCACTTGGCGGCT
TTTGGCGTTATCGTGTTGGTGATTTCCGGGTCGTCTGCGCCATTCAAGATAGCGTTTTGCGCGTCCTTGTAGTGCGTGTT
GGTCACCGGGGTGAGATTTACCGATAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT188583 WP_000126294.1 NZ_CP068824:1221475-1221894 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT188583 NZ_CP090508:c2590343-2590092 [Xylella fastidiosa subsp. morus]
ATGCATCTAAGTGGGGGTTTTGACATGGCGACTTCGATTCGCCTCTCTCCTGAAATGGAGCAAAGGCTGAACTCTCTTGC
CTCCCATACGGGACGCACAAAGGCTTACTACCTGCGTGAAATCATTGAGCATGGCATTGAGGAAATGGAGGATTACTACC
TTGCTGCCGATGTGTTGGAACGTGTCCGTCATGGACAAGAACAGGTGCATTCTGCTGCCGATGTGAGGAAAACGCTTGGC
CTGGACGATTGA

Structures

Toxin

• AlphaFold DB: V0YLE2