Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3807892..3808586 Replicon chromosome
Accession NZ_CP068823
Organism Escherichia coli strain RIVM_C029494

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag JNN66_RS18050 Protein ID WP_001263491.1
Coordinates 3807892..3808290 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag JNN66_RS18055 Protein ID WP_000554755.1
Coordinates 3808293..3808586 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN66_RS18025 3803154..3804611 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
JNN66_RS23350 3804659..3804901 + 243 WP_001029361.1 hypothetical protein -
JNN66_RS18030 3804918..3805427 - 510 WP_001361775.1 hydrolase -
JNN66_RS18035 3805489..3806103 - 615 WP_075862064.1 peptide chain release factor H -
JNN66_RS18040 3806100..3807239 - 1140 WP_170990260.1 RNA ligase RtcB family protein -
JNN66_RS18045 3807430..3807882 - 453 WP_075862062.1 GNAT family N-acetyltransferase -
JNN66_RS18050 3807892..3808290 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JNN66_RS18055 3808293..3808586 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JNN66_RS18060 3808638..3809693 - 1056 WP_075862061.1 DNA polymerase IV -
JNN66_RS18065 3809764..3810549 - 786 WP_179145761.1 putative lateral flagellar export/assembly protein LafU -
JNN66_RS18070 3810521..3812233 + 1713 Protein_3538 flagellar biosynthesis protein FlhA -
JNN66_RS18075 3812345..3812842 - 498 WP_000006244.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3807892..3824048 16156


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T188569 WP_001263491.1 NZ_CP068823:c3808290-3807892 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T188569 NZ_CP090508:c483995-483699 [Xylella fastidiosa subsp. morus]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCAGCCATCGC
CTCGCGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCCGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCGGCGTGGCGACACGATCTACTTGCTGCTTTGCGGCGGTGACAAAGGA
TCACAAGCGCGCGACATCAAGACTGCGCTGCACCTGTCTGAACAATGGAGCGAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT188569 WP_000554755.1 NZ_CP068823:c3808586-3808293 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT188569 NZ_CP090508:c483696-483403 [Xylella fastidiosa subsp. morus]
ATGACCGAGAAACTGACGAGCTACGATCCAGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCTGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACAACATTGGCC
GTGATGAAGGCGCTCGGGATCGAGCTGTCTGCGAAACCGTCTGGTGTTCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References