Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2438626..2439188 Replicon chromosome
Accession NZ_CP068823
Organism Escherichia coli strain RIVM_C029494

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag JNN66_RS11415 Protein ID WP_000605676.1
Coordinates 2438910..2439188 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JNN66_RS11410 Protein ID WP_000781370.1
Coordinates 2438626..2438910 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN66_RS11395 2433985..2437032 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
JNN66_RS11400 2437045..2437929 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
JNN66_RS11405 2437922..2438575 + 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
JNN66_RS11410 2438626..2438910 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
JNN66_RS11415 2438910..2439188 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JNN66_RS11420 2439374..2440384 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
JNN66_RS11425 2440518..2442215 - 1698 WP_000433464.1 malate dehydrogenase -
JNN66_RS11430 2442372..2442509 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
JNN66_RS11435 2442611..2442826 - 216 WP_000495766.1 biofilm-dependent modulation protein -
JNN66_RS11440 2443171..2443602 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T188556 WP_000605676.1 NZ_CP068823:c2439188-2438910 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T188556 NZ_CP090506:c1154845-1154441 [Xylella fastidiosa subsp. morus]
GTGAGCGGCTATATGCTCGACACGAATATCATCAGCGATATCATTCGTAACCCATTCGGGGCGGTTGCTTGCCGTATTGA
ACACGTTGGGTATCCGAACATTTGCACGAGCGTTATCGTGGCGGCTGAACTTCGCTATGGATGCACGAAAAATGGCTCGG
TAAAGCTGCTGAGCAGGGTACAGGACATTCTTAAAACGCTGCCGCTCCTGCCTTTGGAAATACCTGTTGACACAACATAT
GGCAGTATCCGTGCTGAGCTGGAGGCTGCGGGTCAGCTGATCGGCGCTAATGATCTATTGATTGCTGCGCATGCTTACGT
ACTCGGGCTGACTTTGGTCACGGACAACACCCGCGAGTTCAGCCGAATTCGCGGCTTGGATGTGCAGAACTGGCTAGAGA
GGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT188556 WP_000781370.1 NZ_CP068823:c2438910-2438626 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT188556 NZ_CP090506:c1155129-1154842 [Xylella fastidiosa subsp. morus]
ATGGATGCCGTGCCGATGCCAATTTCATCTCCGTTATCACGTACCAAGGAAGCGAAGCTATTCCGAAATAATCGTAGCCA
GGCGGTACGAATTCCCGTTGAATTCGAGCTGCCGGGCGATCGTGTTCTGATCCACCGTGAGGGGAGCAAGCTCATTATTG
AGCCGATCACCAGGCCAACCAATATTGTTGAATTACTGCACGAGTGGCGAAGAGAAACACCACTCGACCCAGAAGATCAA
TTCCCTGATATTGATGACATGCCCGTTCAGCCAGAGGATATTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References