Detailed information of TA system TA188551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 673850..674649
• Replicon: chromosome
• Accession: NZ_CP068823
• Organism: Escherichia coli strain RIVM_C029494

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: JNN66_RS03260
• Protein ID: WP_000347273.1
• Coordinates: 673850..674314 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: JNN66_RS03265
• Protein ID: WP_001308975.1
• Coordinates: 674314..674649 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN66_RS03230	668851..669285	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
JNN66_RS03235	669303..670181	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JNN66_RS03240	670171..670950	-	780	WP_075862220.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
JNN66_RS03245	670961..671434	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JNN66_RS03250	671457..672737	-	1281	WP_000681958.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JNN66_RS03255	672986..673795	+	810	WP_000072180.1	aga operon transcriptional regulator AgaR	-
JNN66_RS03260	673850..674314	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JNN66_RS03265	674314..674649	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JNN66_RS03270	674798..676369	-	1572	WP_001273755.1	galactarate dehydratase	-
JNN66_RS03275	676744..678078	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
JNN66_RS03280	678094..678864	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T188551 WP_000347273.1 NZ_CP068823:c674314-673850 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T188551 NZ_CP090506:c530185-529877 [Xylella fastidiosa subsp. morus]
GTGACCATCACAAAGAAAATCAATGTCTCCGAACTGCCAGAGTTCGATGCCGCCGAATACCTGAACAGCGAAGAGGAGGT
AGCCGCGTATCTCACGGCTGTTCTTGAAGAAAACGATCCAGCACTGTTAGCGGCTGCGCTGGGTGACATTGCCCGTTCAC
GTGGTATGTCTCAGATTGCGAAGGATTCTGGCATCACGCGGGAAGCTCTATACAAGGCTCTTCGGCCTGGCAGTGAACCA
CGCTTCGATACGATAAGCCGCGTCTGTACCGCCCTAGGTATACGTCTAGTAGCGCAACCAATGCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT188551 WP_001308975.1 NZ_CP068823:c674649-674314 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT188551 NZ_CP090506:c530488-530189 [Xylella fastidiosa subsp. morus]
ATGACCTACACGGTAAAACGACTTGAAGGGTTCTCTGACTGGCTAAAGGGCCTAAAGGATGGGCTGGCAAGGCAGCGCCT
CATCAAGCGCTTACGCAAGGTGCAGCTCGGCAACTTTGGAGACGTGCAGCCAGTGGGTGAAGGCGTGTTTGAAATGCGCG
AACACTTCGGACCTGGCTGGCGTATGTACTACGTGCAGCGAGGCAGCTTTCTCATCGTGATGCTCGGCGGCGGCGACAAG
TCAACGCAGCAATCAGACATTCGTCGGGCCATTGAGCTAGCAAAATCTTTAGAGGATTGA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08