Detailed information of TA system TA188547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4977108..4977710
• Replicon: chromosome
• Accession: NZ_CP068822
• Organism: Escherichia coli strain RIVM_C029324

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN44_RS23725
• Protein ID: WP_000897305.1
• Coordinates: 4977399..4977710 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN44_RS23720
• Protein ID: WP_000356397.1
• Coordinates: 4977108..4977398 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN44_RS23700	4973610..4974512	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN44_RS23705	4974509..4975144	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN44_RS23710	4975141..4976070	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN44_RS23715	4976285..4976503	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
JNN44_RS23720	4977108..4977398	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
JNN44_RS23725	4977399..4977710	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN44_RS23730	4977939..4978847	+	909	WP_001298596.1	alpha/beta hydrolase	-
JNN44_RS23735	4978911..4979852	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN44_RS23740	4979897..4980334	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JNN44_RS23745	4980331..4981203	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JNN44_RS23750	4981197..4981796	-	600	WP_001298594.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T188547 WP_000897305.1 NZ_CP068822:c4977710-4977399 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188547 NZ_CP090506:c479536-479237 [Xylella fastidiosa subsp. morus]
ATGACCTACACGGTAAAACGACTTGAAGGGTTCTCTGACTGGCTAAAGGGCCTAAAGGATGGGCTGGCAAGGCAGCGCCT
CATCAAGCGCTTACGCAAGGTGCAGCTCGGCAACTTTGGAGACGTGCAGCCAGTGGGTGAAGGCGTGTTTGAAATGCGCG
AACACTTCGGACCTGGCTGGCGTATGTACTACGTGCAGCGAGGCAGCTTTCTCATCGTGATGCTCGGCGGCGGCGACAAG
TCAACGCAGCAATCAGACATTCGTCGGGCCATTGAGCTAGCAAAATCTTTAGAGGATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT188547 WP_000356397.1 NZ_CP068822:c4977398-4977108 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188547 NZ_CP090506:c479233-478925 [Xylella fastidiosa subsp. morus]
GTGACCATCACAAAGAAAATCAATGTCTCCGAACTGCCAGAGTTCGATGCCGCCGAATACCTGAACAGCGAAGAGGAGGT
AGCCGCGTATCTCACGGCTGTTCTTGAAGAAAACGATCCAGCACTGTTAGCGGCTGCGCTGGGTGACATTGCCCGTTCAC
GTGGTATGTCTCAGATTGCGAAGGATTCTGGCATCACGCGGGAAGCTCTATACAAGGCTCTTCGGCCTGGCAGTGAACCA
CGCTTCGATACGATAAGCCGCGTCTGTACCGCCCTAGGTATACGTCTAGTAGCGCAACCAATGCATTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7