Detailed information of TA system TA188541

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4368260..4368672
• Replicon: chromosome
• Accession: NZ_CP068822
• Organism: Escherichia coli strain RIVM_C029324

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: JNN44_RS20920
• Protein ID: WP_000132614.1
• Coordinates: 4368331..4368672 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4368260..4368336 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN44_RS20910	4364935..4366404	+	1470	WP_001540814.1	type I restriction-modification system subunit M	-
JNN44_RS20915	4366404..4368110	+	1707	WP_001100194.1	restriction endonuclease subunit S	-
-	4368260..4368336	-	77	NuclAT_6	-	Antitoxin
-	4368260..4368336	-	77	NuclAT_6	-	Antitoxin
-	4368260..4368336	-	77	NuclAT_6	-	Antitoxin
-	4368260..4368336	-	77	NuclAT_6	-	Antitoxin
-	4368260..4368336	-	77	NuclAT_7	-	Antitoxin
-	4368260..4368336	-	77	NuclAT_7	-	Antitoxin
-	4368260..4368336	-	77	NuclAT_7	-	Antitoxin
-	4368260..4368336	-	77	NuclAT_7	-	Antitoxin
JNN44_RS20920	4368331..4368672	+	342	WP_000132614.1	endoribonuclease SymE	Toxin
JNN44_RS20925	4368719..4370812	-	2094	WP_000648237.1	DUF262 domain-containing protein	-
JNN44_RS20930	4370910..4370990	-	81	WP_020233658.1	hypothetical protein	-
JNN44_RS20935	4371218..4372138	-	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
JNN44_RS20940	4372323..4373603	+	1281	WP_001298033.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	4352139..4370875	18736

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T188541 WP_000132614.1 NZ_CP068822:4368331-4368672 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T188541 NZ_CP090504:1773072-1773374 [Xylella fastidiosa subsp. morus]
ATGGAGAAAGGCACACCCCACTACAAGCTACCAGTCGTCAAAGCCTTGGTGAAAGCTGGCAAGGTCAGGGCTACAGCGAC
CGCCTACCAAGGAGCACGTGAGCTTGGCATCAAAGATTTAACTGGAATGTGCGCCGTTGTCTTGGCGCTCACGTCTGCCG
ACTTCTACAAGAGTATGACGACCTACACGGATCACACATTATTCCAGGATGTGTACCACGCCAAGACGGCAAGCGGTGAT
GAGGTGTACCTGAAGTTAACTGTCATTGATGACGTGTTGATTGTTTCCTTCAAGGAGTTATGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT188541 NZ_CP068822:c4368336-4368260 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin