Detailed information of TA system TA188537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3974646..3975340
• Replicon: chromosome
• Accession: NZ_CP068822
• Organism: Escherichia coli strain RIVM_C029324

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: JNN44_RS19035
• Protein ID: WP_001263500.1
• Coordinates: 3974646..3975044 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: JNN44_RS19040
• Protein ID: WP_000554758.1
• Coordinates: 3975047..3975340 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN44_RS19010	3970011..3970469	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
JNN44_RS19015	3970730..3972187	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
JNN44_RS19020	3972244..3972858	-	615	WP_000602124.1	peptide chain release factor H	-
JNN44_RS19025	3972855..3973994	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
JNN44_RS19030	3974184..3974636	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
JNN44_RS19035	3974646..3975044	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN44_RS19040	3975047..3975340	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN44_RS19045	3975392..3976447	-	1056	WP_001226168.1	DNA polymerase IV	-
JNN44_RS19050	3976518..3977303	-	786	WP_000207578.1	putative lateral flagellar export/assembly protein LafU	-
JNN44_RS19055	3977275..3978987	+	1713	Protein_3738	flagellar biosynthesis protein FlhA	-
JNN44_RS19060	3979307..3979804	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3974646..3995000	20354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T188537 WP_001263500.1 NZ_CP068822:c3975044-3974646 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188537 NZ_CP090504:c1491303-1491016 [Xylella fastidiosa subsp. morus]
ATGGCTAAGTCCTATCGACTGACACCGCTGGCCGAGGCCGATCTTGAAGAAATCTGGTTCTACACATTCAGGCACTGGTC
GATAGAGCAGGCCGACAGCTACCACCGTAGCCTAGTTGCAGTTTTCGAGGGGCTGGCAGCAGGTACAAAGCAAGGCTGCC
CTTCTGTTTTGACGGACTTCAATAAATACCTATGTGGTTCGCATGTAGTGTATTTCATGGATTACGCCGATCACTTGGAT
ATTATCCGCATCCTGCATCAGCGGCAGGATACCGAGCGGTACCTATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT188537 WP_000554758.1 NZ_CP068822:c3975340-3975047 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188537 NZ_CP090504:c1491538-1491296 [Xylella fastidiosa subsp. morus]
ATGAGTCGAAACACATCCGTTGCCCTCGGGCCGCACTTCACCAGCTTTATTGATGCCCAAGTCCAGGGCGGTCGCTACGG
TACCGCCAGCGATGTTGTCAGGGCCGGTCTGCGGCTGCTTGAGGAACACGAAACAAAGGTGAAGGCACTGCAAGATGCGC
TGAATGTTGGCCACCAATCCGGCGAGCCGCGCCCGTTCGACAGCGAGGTGTTTTTGAGCCGGATGCACACGCAGCATGGC
TAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3