Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2585463..2586025 Replicon chromosome
Accession NZ_CP068822
Organism Escherichia coli strain RIVM_C029324

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag JNN44_RS12200 Protein ID WP_000605675.1
Coordinates 2585463..2585741 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JNN44_RS12205 Protein ID WP_000781370.1
Coordinates 2585741..2586025 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN44_RS12175 2581049..2581480 - 432 WP_000152305.1 peroxiredoxin OsmC -
JNN44_RS12180 2581825..2582040 + 216 WP_000495766.1 biofilm-dependent modulation protein -
JNN44_RS12185 2582142..2582279 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
JNN44_RS12190 2582436..2584133 + 1698 WP_000433462.1 malate dehydrogenase -
JNN44_RS12195 2584267..2585277 + 1011 WP_000642380.1 alcohol dehydrogenase AdhP -
JNN44_RS12200 2585463..2585741 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JNN44_RS12205 2585741..2586025 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
JNN44_RS12210 2586254..2586907 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
JNN44_RS12215 2586900..2587784 - 885 WP_001240581.1 formate dehydrogenase N subunit beta -
JNN44_RS12220 2587797..2590844 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T188531 WP_000605675.1 NZ_CP068822:2585463-2585741 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T188531 NZ_CP090504:c530215-529907 [Xylella fastidiosa subsp. morus]
GTGACCATCACAAAGAAAATCAATGTCTCCGAACTGCCAGAGTTCGATGCCGCCGAATACCTGAACAGCGAAGAGGAGGT
AGCCGCGTATCTCACGGCTGTTCTTGAAGAAAACGATCCAGCACTGTTAGCGGCTGCGCTGGGTGACATTGCCCGTTCAC
GTGGTATGTCTCAGATTGCGAAGGATTCTGGCATCACGCGGGAAGCTCTATACAAGGCTCTTCGGCCTGGCAGTGAACCA
CGCTTCGATACGATAAGCCGCGTCTGTACCGCCCTAGGTATACGTCTAGTAGCGCAACCAATGCATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT188531 WP_000781370.1 NZ_CP068822:2585741-2586025 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT188531 NZ_CP090504:c530518-530219 [Xylella fastidiosa subsp. morus]
ATGACCTACACGGTAAAACGACTTGAAGGGTTCTCTGACTGGCTAAAGGGCCTAAAGGATGGGCTGGCAAGGCAGCGCCT
CATCAAGCGCTTACGCAAGGTGCAGCTCGGCAACTTTGGAGACGTGCAGCCAGTGGGTGAAGGCGTGTTTGAAATGCGCG
AACACTTCGGACCTGGCTGGCGTATGTACTACGTGCAGCGAGGCAGCTTTCTCATCGTGATGCTCGGCGGCGGCGACAAG
TCAACGCAGCAATCAGACATTCGTCGGGCCATTGAGCTAGCAAAATCTTTAGAGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References