Detailed information of TA system TA188513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5077837..5078439
• Replicon: chromosome
• Accession: NZ_CP068821
• Organism: Escherichia coli strain RIVM_C029952

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN43_RS24625
• Protein ID: WP_000897305.1
• Coordinates: 5078128..5078439 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN43_RS24620
• Protein ID: WP_000356395.1
• Coordinates: 5077837..5078127 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN43_RS24585	5073461..5074363	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN43_RS24590	5074360..5074995	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN43_RS24595	5074992..5075921	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN43_RS24600	5076103..5076345	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN43_RS24605	5076564..5076782	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN43_RS24610	5077201..5077479	-	279	WP_001315112.1	hypothetical protein	-
JNN43_RS24615	5077531..5077752	-	222	WP_001550354.1	hypothetical protein	-
JNN43_RS24620	5077837..5078127	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN43_RS24625	5078128..5078439	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN43_RS24630	5078668..5079576	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN43_RS24635	5079744..5081558	-	1815	WP_001550352.1	hypothetical protein	-
JNN43_RS24640	5081974..5082915	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN43_RS24645	5082960..5083397	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T188513 WP_000897305.1 NZ_CP068821:c5078439-5078128 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188513 NZ_CP090484:c2792443-2792144 [Burkholderia sp. S-53]
ATGGACAAGATCAGCACCCGGCCGTGGGATTCGGCCGACCATCTGAAAACCGAAGACGACATGGCCGACTACTTCGAGGC
CTGCCTGCAAGAGGCCGGCGACGATCCGGCCTTCATCGCGCACGCGCTCGGCGTGATCGCACGCGCCCGCGGCATGTCGC
AGGTCGCGCGCGATGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAACCCGAGCTTCGGCACG
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAACTGCACGGCTCGAAAGCGCACGCGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT188513 WP_000356395.1 NZ_CP068821:c5078127-5077837 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188513 NZ_CP090484:c2792764-2792447 [Burkholderia sp. S-53]
ATGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGACGCATGGTTTGCCGGCCTGCAGGATCGCGT
CGCGAAGCGCCGCATCCAGGCGCGCATCGACCGCCTGTCGATGGGCAATCCGGGCGACTGGAAATCCGCCGGCTCGCCGG
TCGTCGAAATGCGTATCGACCATGGCCCCGGTTATCGCGTCTACTACGTCCGGCGCGGAACGATCTGGGTCATCCTGCTC
TGCGGCGGCGATAAATCGACGCAACAGGCCGACATACGCGCCGCGCACGCGATGCTTGCGCATCTCGACATGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7