Detailed information of TA system TA188506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4051644..4052338
• Replicon: chromosome
• Accession: NZ_CP068821
• Organism: Escherichia coli strain RIVM_C029952

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: JNN43_RS19590
• Protein ID: WP_001263491.1
• Coordinates: 4051644..4052042 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: JNN43_RS19595
• Protein ID: WP_000554755.1
• Coordinates: 4052045..4052338 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN43_RS19560	4046813..4048057	-	1245	WP_000189541.1	esterase FrsA	-
-	4047473..4047553	-	81	NuclAT_11	-	-
-	4047473..4047553	-	81	NuclAT_11	-	-
-	4047473..4047553	-	81	NuclAT_11	-	-
-	4047473..4047553	-	81	NuclAT_11	-	-
JNN43_RS19565	4048149..4048607	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JNN43_RS19570	4048868..4050325	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JNN43_RS19575	4050382..4050734	-	353	Protein_3830	peptide chain release factor H	-
JNN43_RS19580	4050730..4050936	-	207	Protein_3831	RtcB family protein	-
JNN43_RS19585	4051182..4051634	-	453	WP_019842500.1	GNAT family N-acetyltransferase	-
JNN43_RS19590	4051644..4052042	-	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN43_RS19595	4052045..4052338	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN43_RS19600	4052390..4053445	-	1056	WP_001550655.1	DNA polymerase IV	-
JNN43_RS19605	4053516..4054301	-	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
JNN43_RS19610	4054273..4055985	+	1713	Protein_3837	flagellar biosynthesis protein FlhA	-
JNN43_RS19615	4056090..4056368	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JNN43_RS19620	4056361..4056717	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4041580..4052338	10758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T188506 WP_001263491.1 NZ_CP068821:c4052042-4051644 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188506 NZ_CP090484:c382624-382208 [Burkholderia sp. S-53]
GTGTTTTTGATTGATACGAACGTCATCAGCGAAATCAGGAAGGGCAAGCGAACCAACCGCGGCGTGCGAGCGTTCTTCAG
GCAGGCCGAGGCCGACGCGAGCCCGCTTTACCTGTCCGTTGTGACAGTGGCCGAACTTCGGCGCGGCGTCGACCTGATCC
GTCATCGCGGCGATCACCGCCAGGCGTCGGCGCTCGAGGCTTGGATGGCGACGATCCTGTCCGGCTATGCGCCGAACCTC
CTGCCGGTCGATATCGAGATCAGCCAGATGTGGGGGCATTTGCGTGTGCCCGATCCGACGCACGAACTCGACAAACTGAT
CGCGGCCACTGCGCTGATCAACGATCTTACGGTCGTCACGCGCAATGTCGACGATTTCGCTCGCACCGGTGTCCGGCTGC
TGAACCCGTTCGACTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT188506 WP_000554755.1 NZ_CP068821:c4052338-4052045 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188506 NZ_CP090484:c382847-382611 [Burkholderia sp. S-53]
ATGGCAAATCTTCTGGTGCGTAACGTGGATGACATTCTCGTTCAGAGCCTGCGCGAGCAGGCTGCGGCAAATGGCAGGAG
TGCCGAGGCCGAACATCGGGCCATTCTGGCCGATGCGCTCGGCCGGCCGAAGCGAAGGACATTCGCGCAAGTGCTGATGA
GCATGCCCGATGTTGGCGAAGACACGGATTTCCAACGTGTTCAGGATTCCGGCGAGGTCAGGCGTGTTTTTGATTGA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5