Detailed information of TA system TA188490

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2754416..2754787
• Replicon: chromosome
• Accession: NZ_CP068821
• Organism: Escherichia coli strain RIVM_C029952

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: JNN43_RS13275
• Protein ID: WP_001317028.1
• Coordinates: 2754593..2754787 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2754416..2754594 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN43_RS13250	2750371..2751744	+	1374	WP_001551034.1	ATP-dependent RNA helicase DbpA	-
JNN43_RS13255	2751873..2752808	-	936	WP_000662472.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
JNN43_RS13260	2752860..2754095	-	1236	WP_001551035.1	site-specific integrase	-
JNN43_RS13265	2754097..2754312	-	216	WP_000079604.1	excisionase XisR	-
JNN43_RS13270	2754412..2754600	-	189	WP_001551036.1	DUF1187 family protein	-
-	2754416..2754594	+	179	NuclAT_0	-	Antitoxin
-	2754416..2754594	+	179	NuclAT_0	-	Antitoxin
-	2754416..2754594	+	179	NuclAT_0	-	Antitoxin
-	2754416..2754594	+	179	NuclAT_0	-	Antitoxin
JNN43_RS13275	2754593..2754787	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
JNN43_RS13280	2754844..2755653	-	810	WP_001551037.1	recombination protein RecT	-
JNN43_RS13285	2755646..2758297	-	2652	WP_001551038.1	exodeoxyribonuclease VIII	-
JNN43_RS13290	2758399..2758674	-	276	WP_001314664.1	hypothetical protein	-
JNN43_RS13295	2758749..2758919	-	171	WP_001551041.1	phage protein	-
JNN43_RS13300	2758919..2759140	-	222	WP_000560221.1	killing protein KilR	-
JNN43_RS13305	2759561..2759713	-	153	WP_001551042.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2752860..2800153	47293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T188490 WP_001317028.1 NZ_CP068821:c2754787-2754593 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T188490 NZ_CP090468:c5262777-5262394 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT188490 NZ_CP068821:2754416-2754594 [Escherichia coli]
GAGGACTGAAGTCTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGGCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin