Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1201555..1202282 Replicon chromosome
Accession NZ_CP068821
Organism Escherichia coli strain RIVM_C029952

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag JNN43_RS05785 Protein ID WP_000547555.1
Coordinates 1201555..1201866 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JNN43_RS05790 Protein ID WP_000126294.1
Coordinates 1201863..1202282 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN43_RS05760 1197490..1199199 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
JNN43_RS05765 1199209..1199751 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
JNN43_RS05770 1199751..1200518 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
JNN43_RS05775 1200515..1200925 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
JNN43_RS05780 1200918..1201388 + 471 WP_001551542.1 hydrogenase maturation peptidase HycI -
JNN43_RS05785 1201555..1201866 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
JNN43_RS05790 1201863..1202282 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
JNN43_RS05795 1202361..1203785 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
JNN43_RS05800 1203794..1205251 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
JNN43_RS05805 1205511..1206521 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
JNN43_RS05810 1206670..1207197 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T188483 WP_000547555.1 NZ_CP068821:1201555-1201866 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T188483 NZ_CP090468:1994590-1994692 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT188483 WP_000126294.1 NZ_CP068821:1201863-1202282 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT188483 NZ_CP090468:c1994699-1994554 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References