Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 670880..671679 Replicon chromosome
Accession NZ_CP068821
Organism Escherichia coli strain RIVM_C029952

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag JNN43_RS03215 Protein ID WP_000347270.1
Coordinates 670880..671344 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag JNN43_RS03220 Protein ID WP_001551693.1
Coordinates 671344..671679 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN43_RS03185 665881..666315 - 435 WP_000948837.1 PTS sugar transporter subunit IIA -
JNN43_RS03190 666333..667211 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JNN43_RS03195 667201..667980 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JNN43_RS03200 667991..668464 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JNN43_RS03205 668487..669767 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
JNN43_RS03210 670016..670825 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JNN43_RS03215 670880..671344 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JNN43_RS03220 671344..671679 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JNN43_RS03225 671828..673399 - 1572 WP_001273738.1 galactarate dehydratase -
JNN43_RS03230 673774..675108 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
JNN43_RS03235 675124..675894 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T188479 WP_000347270.1 NZ_CP068821:c671344-670880 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T188479 NZ_CP090467:c1494-1228 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT188479 WP_001551693.1 NZ_CP068821:c671679-671344 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT188479 NZ_CP090467:c1702-1478 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References