Detailed information of TA system TA188476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4547896..4548623
• Replicon: chromosome
• Accession: NZ_CP068820
• Organism: Escherichia coli strain RIVM_C029951

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: JNN41_RS21960
• Protein ID: WP_000547555.1
• Coordinates: 4547896..4548207 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN41_RS21965
• Protein ID: WP_000126294.1
• Coordinates: 4548204..4548623 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN41_RS21935	4543831..4545540	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
JNN41_RS21940	4545550..4546092	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
JNN41_RS21945	4546092..4546859	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
JNN41_RS21950	4546856..4547266	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
JNN41_RS21955	4547259..4547729	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
JNN41_RS21960	4547896..4548207	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
JNN41_RS21965	4548204..4548623	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
JNN41_RS21970	4548702..4550126	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
JNN41_RS21975	4550135..4551592	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
JNN41_RS21980	4551852..4552862	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
JNN41_RS21985	4553011..4553538	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T188476 WP_000547555.1 NZ_CP068820:4547896-4548207 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T188476 NZ_CP090463:c88925-88482 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT188476 WP_000126294.1 NZ_CP068820:4548204-4548623 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT188476 NZ_CP090463:c89152-88922 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: V0YLE2