Detailed information of TA system TA188469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3081536..3082138
• Replicon: chromosome
• Accession: NZ_CP068820
• Organism: Escherichia coli strain RIVM_C029951

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN41_RS14870
• Protein ID: WP_000897305.1
• Coordinates: 3081827..3082138 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN41_RS14865
• Protein ID: WP_000356395.1
• Coordinates: 3081536..3081826 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN41_RS14830	3077160..3078062	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN41_RS14835	3078059..3078694	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN41_RS14840	3078691..3079620	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN41_RS14845	3079802..3080044	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN41_RS14850	3080263..3080481	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN41_RS14855	3080900..3081178	-	279	WP_001315112.1	hypothetical protein	-
JNN41_RS14860	3081230..3081451	-	222	WP_001550354.1	hypothetical protein	-
JNN41_RS14865	3081536..3081826	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN41_RS14870	3081827..3082138	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN41_RS14875	3082367..3083275	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN41_RS14880	3083443..3085257	-	1815	WP_001550352.1	hypothetical protein	-
JNN41_RS14885	3085673..3086614	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN41_RS14890	3086659..3087096	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T188469 WP_000897305.1 NZ_CP068820:c3082138-3081827 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188469 NZ_CP090462:4198742-4198960 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT188469 WP_000356395.1 NZ_CP068820:c3081826-3081536 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188469 NZ_CP090462:4198341-4198715 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7