Detailed information of TA system TA188462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2089770..2090464
• Replicon: chromosome
• Accession: NZ_CP068820
• Organism: Escherichia coli strain RIVM_C029951

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: JNN41_RS10175
• Protein ID: WP_001263491.1
• Coordinates: 2089770..2090168 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: JNN41_RS10180
• Protein ID: WP_000554755.1
• Coordinates: 2090171..2090464 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN41_RS10145	2084939..2086183	-	1245	WP_000189541.1	esterase FrsA	-
-	2085599..2085679	-	81	NuclAT_10	-	-
-	2085599..2085679	-	81	NuclAT_10	-	-
-	2085599..2085679	-	81	NuclAT_10	-	-
-	2085599..2085679	-	81	NuclAT_10	-	-
JNN41_RS10150	2086275..2086733	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JNN41_RS10155	2086994..2088451	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JNN41_RS10160	2088508..2088860	-	353	Protein_1995	peptide chain release factor H	-
JNN41_RS10165	2088856..2089062	-	207	Protein_1996	RtcB family protein	-
JNN41_RS10170	2089308..2089760	-	453	WP_019842500.1	GNAT family N-acetyltransferase	-
JNN41_RS10175	2089770..2090168	-	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN41_RS10180	2090171..2090464	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN41_RS10185	2090516..2091571	-	1056	WP_001550655.1	DNA polymerase IV	-
JNN41_RS10190	2091642..2092427	-	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
JNN41_RS10195	2092399..2094111	+	1713	Protein_2002	flagellar biosynthesis protein FlhA	-
JNN41_RS10200	2094216..2094494	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JNN41_RS10205	2094487..2094843	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2079706..2090464	10758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T188462 WP_001263491.1 NZ_CP068820:c2090168-2089770 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188462 NZ_CP090458:83345-83650 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT188462 WP_000554755.1 NZ_CP068820:c2090464-2090171 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188462 NZ_CP090458:83125-83343 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5