Detailed information of TA system TA188446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4985910..4986512
• Replicon: chromosome
• Accession: NZ_CP068819
• Organism: Escherichia coli strain RIVM_C029042

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN65_RS24055
• Protein ID: WP_000897305.1
• Coordinates: 4986201..4986512 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN65_RS24050
• Protein ID: WP_000356395.1
• Coordinates: 4985910..4986200 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN65_RS24015	4981534..4982436	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN65_RS24020	4982433..4983068	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN65_RS24025	4983065..4983994	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN65_RS24030	4984176..4984418	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN65_RS24035	4984637..4984855	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN65_RS24040	4985274..4985552	-	279	WP_001315112.1	hypothetical protein	-
JNN65_RS24045	4985604..4985825	-	222	WP_001550354.1	hypothetical protein	-
JNN65_RS24050	4985910..4986200	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN65_RS24055	4986201..4986512	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN65_RS24060	4986741..4987649	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN65_RS24065	4987817..4989631	-	1815	WP_001550352.1	hypothetical protein	-
JNN65_RS24070	4990047..4990988	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN65_RS24075	4991033..4991470	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T188446 WP_000897305.1 NZ_CP068819:c4986512-4986201 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188446 NZ_CP090456:c1235304-1234975 [Escherichia coli]
ATGAATACTTTACCTGCAACAACCCAGCGGGCGGCGAAACCCTGCCTGTCACCCGTGGCTGTCTGGAAAATGCTACTGAC
ACACCTGCTGGAACAACACTATGGTCTGACCCTCAACGACACACCGTTCAGTGAGGAACGAGTTATACAGGAACATATCG
ATGCTGGTATCACGCTGGCTGATACTGTGAATTTTCTGGTGGAGAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAGACTCCGTATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGACTTGCTAAAAACTAA
CGTGAAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT188446 WP_000356395.1 NZ_CP068819:c4986200-4985910 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188446 NZ_CP090456:c1235642-1235325 [Escherichia coli]
ATGAGCAATATCACATGGGGCCTGCAGCGGGCTATCACGCCGCGCCTGGGAGCCCGTCTGGTGCAGGAGGGTAACCGACT
GCACTACCTGGCTGACCGGGCCAGCATCACCGGTAGATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAAGTCAGCCCCCAACATGCCCACTGCGTCATCCTGTACCAC
AACGGTTTCACCTGTGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7