Detailed information of TA system TA188439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3984956..3985650
• Replicon: chromosome
• Accession: NZ_CP068819
• Organism: Escherichia coli strain RIVM_C029042

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: JNN65_RS19225
• Protein ID: WP_001263491.1
• Coordinates: 3984956..3985354 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: JNN65_RS19230
• Protein ID: WP_000554755.1
• Coordinates: 3985357..3985650 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN65_RS19195	3980125..3981369	-	1245	WP_000189541.1	esterase FrsA	-
-	3980785..3980865	-	81	NuclAT_10	-	-
-	3980785..3980865	-	81	NuclAT_10	-	-
-	3980785..3980865	-	81	NuclAT_10	-	-
-	3980785..3980865	-	81	NuclAT_10	-	-
JNN65_RS19200	3981461..3981919	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JNN65_RS19205	3982180..3983637	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JNN65_RS19210	3983694..3984046	-	353	Protein_3762	peptide chain release factor H	-
JNN65_RS19215	3984042..3984248	-	207	Protein_3763	RtcB family protein	-
JNN65_RS19220	3984494..3984946	-	453	WP_019842500.1	GNAT family N-acetyltransferase	-
JNN65_RS19225	3984956..3985354	-	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN65_RS19230	3985357..3985650	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN65_RS19235	3985702..3986757	-	1056	WP_001550655.1	DNA polymerase IV	-
JNN65_RS19240	3986828..3987613	-	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
JNN65_RS19245	3987585..3989297	+	1713	Protein_3769	flagellar biosynthesis protein FlhA	-
JNN65_RS19250	3989402..3989680	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JNN65_RS19255	3989673..3990029	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3974892..3985650	10758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T188439 WP_001263491.1 NZ_CP068819:c3985354-3984956 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188439 NZ_CP090452:7314-7592 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGT
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTGCCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCCGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT188439 WP_000554755.1 NZ_CP068819:c3985650-3985357 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188439 NZ_CP090452:7045-7314 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCAAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5