188419

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 671631..672430 Replicon chromosome
Accession NZ_CP068819
Organism Escherichia coli strain RIVM_C029042

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag JNN65_RS03225 Protein ID WP_000347270.1
Coordinates 671631..672095 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag JNN65_RS03230 Protein ID WP_001551693.1
Coordinates 672095..672430 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN65_RS03195 666632..667066 - 435 WP_000948837.1 PTS sugar transporter subunit IIA -
JNN65_RS03200 667084..667962 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JNN65_RS03205 667952..668731 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JNN65_RS03210 668742..669215 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JNN65_RS03215 669238..670518 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
JNN65_RS03220 670767..671576 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JNN65_RS03225 671631..672095 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JNN65_RS03230 672095..672430 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JNN65_RS03235 672579..674150 - 1572 WP_001273738.1 galactarate dehydratase -
JNN65_RS03240 674525..675859 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
JNN65_RS03245 675875..676645 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T188419 WP_000347270.1 NZ_CP068819:c672095-671631 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T188419 NZ_CP090448:993545-993880 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAAGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCTTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACACTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT188419 WP_001551693.1 NZ_CP068819:c672430-672095 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT188419 NZ_CP090448:993297-993545 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATTCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGGCATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References