Detailed information of TA system TA188416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5059550..5060152
• Replicon: chromosome
• Accession: NZ_CP068818
• Organism: Escherichia coli strain RIVM_C028803

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN48_RS24720
• Protein ID: WP_000897305.1
• Coordinates: 5059841..5060152 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN48_RS24715
• Protein ID: WP_000356395.1
• Coordinates: 5059550..5059840 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN48_RS24680	5055174..5056076	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN48_RS24685	5056073..5056708	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN48_RS24690	5056705..5057634	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN48_RS24695	5057816..5058058	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
JNN48_RS24700	5058277..5058495	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
JNN48_RS24705	5058914..5059192	-	279	WP_001315112.1	hypothetical protein	-
JNN48_RS24710	5059244..5059465	-	222	WP_001550354.1	hypothetical protein	-
JNN48_RS24715	5059550..5059840	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
JNN48_RS24720	5059841..5060152	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN48_RS24725	5060381..5061289	+	909	WP_001550353.1	alpha/beta hydrolase	-
JNN48_RS24730	5061457..5063271	-	1815	WP_001550352.1	hypothetical protein	-
JNN48_RS24735	5063687..5064628	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN48_RS24740	5064673..5065110	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T188416 WP_000897305.1 NZ_CP068818:c5060152-5059841 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188416 NZ_CP090448:123954-124139 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT188416 WP_000356395.1 NZ_CP068818:c5059840-5059550 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188416 NZ_CP090448:124156-124566 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCAAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7