Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 4057295..4057989 | Replicon | chromosome |
| Accession | NZ_CP068818 | ||
| Organism | Escherichia coli strain RIVM_C028803 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q0T7Q5 |
| Locus tag | JNN48_RS19805 | Protein ID | WP_001263491.1 |
| Coordinates | 4057295..4057693 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | L4JHF0 |
| Locus tag | JNN48_RS19810 | Protein ID | WP_000554755.1 |
| Coordinates | 4057696..4057989 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JNN48_RS19775 | 4052464..4053708 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| - | 4053124..4053204 | - | 81 | NuclAT_11 | - | - |
| - | 4053124..4053204 | - | 81 | NuclAT_11 | - | - |
| - | 4053124..4053204 | - | 81 | NuclAT_11 | - | - |
| - | 4053124..4053204 | - | 81 | NuclAT_11 | - | - |
| JNN48_RS19780 | 4053800..4054258 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| JNN48_RS19785 | 4054519..4055976 | + | 1458 | WP_001293009.1 | cytosol nonspecific dipeptidase | - |
| JNN48_RS19790 | 4056033..4056385 | - | 353 | Protein_3870 | peptide chain release factor H | - |
| JNN48_RS19795 | 4056381..4056587 | - | 207 | Protein_3871 | RtcB family protein | - |
| JNN48_RS19800 | 4056833..4057285 | - | 453 | WP_019842500.1 | GNAT family N-acetyltransferase | - |
| JNN48_RS19805 | 4057295..4057693 | - | 399 | WP_001263491.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| JNN48_RS19810 | 4057696..4057989 | - | 294 | WP_000554755.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| JNN48_RS19815 | 4058041..4059096 | - | 1056 | WP_001550655.1 | DNA polymerase IV | - |
| JNN48_RS19820 | 4059167..4059952 | - | 786 | WP_019842510.1 | putative lateral flagellar export/assembly protein LafU | - |
| JNN48_RS19825 | 4059924..4061636 | + | 1713 | Protein_3877 | flagellar biosynthesis protein FlhA | - |
| JNN48_RS19830 | 4061741..4062019 | + | 279 | WP_000598760.1 | type II toxin-antitoxin system HicA family toxin | - |
| JNN48_RS19835 | 4062012..4062368 | + | 357 | WP_001030484.1 | type II toxin-antitoxin system HicB family antitoxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 4047231..4057989 | 10758 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15457.86 Da Isoelectric Point: 8.0949
>T188410 WP_001263491.1 NZ_CP068818:c4057693-4057295 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T188410 NZ_CP090438:c1901507-1901037 [Vibrio maritimus]
ATGTTCTTATATCGTATCGTCAAAAAGAAATTTGCTACTAATATCGAGCAAGTATTCGATGGTGAAGGAGCGAGGCGATA
CGGTGGGCGCTGGAATTCAGCGGGTAACCGTTGTGTTTATCTCGCTACCTCAGAATCACTTGCGATTCTCGAAGTCCTGG
TTCATTTGAATGATACCGAATTACTTAACGACTACGATCTTTATCGCATAGATATCGATGAAGCTTTGATCATAGAACTC
GACCAGGGCTATCTACCAGACGATTGGCAAGAAGACCCTGCGCCATCTTCCACTGCTCAGATTGGTGATGAGTGGCTTGA
GAGCGAGATGAGCTTGTGCTTAAAAATCCCTTCGACAATAGTGCCTAGAGAGTTTAATTGTTTACTCAACTTACAACACG
CAGCTTTCAACTTAGAGGAGCTAGAGGTAGTTAAACTTGCGTTTAACCCTGACCACCGTTTGAGCCGTTGA
ATGTTCTTATATCGTATCGTCAAAAAGAAATTTGCTACTAATATCGAGCAAGTATTCGATGGTGAAGGAGCGAGGCGATA
CGGTGGGCGCTGGAATTCAGCGGGTAACCGTTGTGTTTATCTCGCTACCTCAGAATCACTTGCGATTCTCGAAGTCCTGG
TTCATTTGAATGATACCGAATTACTTAACGACTACGATCTTTATCGCATAGATATCGATGAAGCTTTGATCATAGAACTC
GACCAGGGCTATCTACCAGACGATTGGCAAGAAGACCCTGCGCCATCTTCCACTGCTCAGATTGGTGATGAGTGGCTTGA
GAGCGAGATGAGCTTGTGCTTAAAAATCCCTTCGACAATAGTGCCTAGAGAGTTTAATTGTTTACTCAACTTACAACACG
CAGCTTTCAACTTAGAGGAGCTAGAGGTAGTTAAACTTGCGTTTAACCCTGACCACCGTTTGAGCCGTTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11235.78 Da Isoelectric Point: 5.2170
>AT188410 WP_000554755.1 NZ_CP068818:c4057989-4057696 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT188410 NZ_CP090438:c1901950-1901507 [Vibrio maritimus]
ATGACTGCCTCAACAAAATTTGTTCCTTCTCAATCGACTGCCGGATTTTGGTCGCAAATTGGTGTGCCACCAAGAGGTCG
CAAACTGCATGAGGCGATCGAGGCTGGCTTTGATTATGCGACCTATCAAAAACTCGCCAACGTTACAGGCATTGAGAAAA
AAGAGCTAGCACGTATCACCAACATTAAAAGTGCGACTCTGAGTCGACGAGCGAAGGACGGTAAGTTCTCTTCTGAAGAG
AGTGATAGACTTTATCGCTACGCTGAAGTGTATAACGCGTCTTTGCGACTATTTGAAGATGATAAGTTGAAAGCGTTTCA
ATGGATGCACAATCCAGTTAAAGCTCTCGGGTACGTTGCGCCAAGCGATATGTTGAAAACCAGTGCTGAGACCGAAGAGG
TATTGAGCGTAATCGGTCGCCTTGAACACGGTGTATTCAACTGA
ATGACTGCCTCAACAAAATTTGTTCCTTCTCAATCGACTGCCGGATTTTGGTCGCAAATTGGTGTGCCACCAAGAGGTCG
CAAACTGCATGAGGCGATCGAGGCTGGCTTTGATTATGCGACCTATCAAAAACTCGCCAACGTTACAGGCATTGAGAAAA
AAGAGCTAGCACGTATCACCAACATTAAAAGTGCGACTCTGAGTCGACGAGCGAAGGACGGTAAGTTCTCTTCTGAAGAG
AGTGATAGACTTTATCGCTACGCTGAAGTGTATAACGCGTCTTTGCGACTATTTGAAGATGATAAGTTGAAAGCGTTTCA
ATGGATGCACAATCCAGTTAAAGCTCTCGGGTACGTTGCGCCAAGCGATATGTTGAAAACCAGTGCTGAGACCGAAGAGG
TATTGAGCGTAATCGGTCGCCTTGAACACGGTGTATTCAACTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4P7TPK7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829G9H5 |