Detailed information of TA system TA188387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5005870..5006472
• Replicon: chromosome
• Accession: NZ_CP068817
• Organism: Escherichia coli strain RIVM_C028786

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN59_RS23940
• Protein ID: WP_000897305.1
• Coordinates: 5006161..5006472 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN59_RS23935
• Protein ID: WP_000356397.1
• Coordinates: 5005870..5006160 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN59_RS23915	5002372..5003274	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN59_RS23920	5003271..5003906	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN59_RS23925	5003903..5004832	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN59_RS23930	5005047..5005265	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
JNN59_RS23935	5005870..5006160	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
JNN59_RS23940	5006161..5006472	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN59_RS23945	5006701..5007609	+	909	WP_001298596.1	alpha/beta hydrolase	-
JNN59_RS23950	5007673..5008614	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN59_RS23955	5008659..5009096	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JNN59_RS23960	5009093..5009965	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JNN59_RS23965	5009959..5010558	-	600	WP_001298594.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T188387 WP_000897305.1 NZ_CP068817:c5006472-5006161 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188387 NZ_CP090429:c4937732-4936815 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCAATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT188387 WP_000356397.1 NZ_CP068817:c5006160-5005870 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188387 NZ_CP090429:c4938496-4937738 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7