Detailed information of TA system TA188377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3985347..3986041
• Replicon: chromosome
• Accession: NZ_CP068817
• Organism: Escherichia coli strain RIVM_C028786

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: JNN59_RS19085
• Protein ID: WP_001263500.1
• Coordinates: 3985347..3985745 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: JNN59_RS19090
• Protein ID: WP_000554758.1
• Coordinates: 3985748..3986041 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN59_RS19060	3980712..3981170	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
JNN59_RS19065	3981431..3982888	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
JNN59_RS19070	3982945..3983559	-	615	WP_000602124.1	peptide chain release factor H	-
JNN59_RS19075	3983556..3984695	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
JNN59_RS19080	3984885..3985337	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
JNN59_RS19085	3985347..3985745	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN59_RS19090	3985748..3986041	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN59_RS19095	3986093..3987148	-	1056	WP_001226168.1	DNA polymerase IV	-
JNN59_RS19100	3987219..3988004	-	786	WP_000207578.1	putative lateral flagellar export/assembly protein LafU	-
JNN59_RS19105	3987976..3989688	+	1713	Protein_3747	flagellar biosynthesis protein FlhA	-
JNN59_RS19110	3989716..3989925	+	210	WP_071778446.1	hypothetical protein	-
JNN59_RS19115	3990008..3990505	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	blaOXA-48	gmhA/lpcA	3985347..4023816	38469

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T188377 WP_001263500.1 NZ_CP068817:c3985745-3985347 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188377 NZ_CP090428:c918926-918633 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAGCTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTACGCATTCATTAGGTATTAAACTTGTCCCAACGCACATCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT188377 WP_000554758.1 NZ_CP068817:c3986041-3985748 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188377 NZ_CP090428:c919207-918923 [Pasteurella multocida]
GTGGGTCGGTTAAGGGAGGTAAAAAATTTATCCGCCAAAGCCGCTGTTCTTGCTCGTATTAACAGAGTAATGAATGGTAA
TTTGGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3