Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2621619..2622181 Replicon chromosome
Accession NZ_CP068817
Organism Escherichia coli strain RIVM_C028786

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag JNN59_RS12425 Protein ID WP_000605675.1
Coordinates 2621619..2621897 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JNN59_RS12430 Protein ID WP_000781370.1
Coordinates 2621897..2622181 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN59_RS12400 2617205..2617636 - 432 WP_000152305.1 peroxiredoxin OsmC -
JNN59_RS12405 2617981..2618196 + 216 WP_000495766.1 biofilm-dependent modulation protein -
JNN59_RS12410 2618298..2618435 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
JNN59_RS12415 2618592..2620289 + 1698 WP_000433462.1 malate dehydrogenase -
JNN59_RS12420 2620423..2621433 + 1011 WP_000642380.1 alcohol dehydrogenase AdhP -
JNN59_RS12425 2621619..2621897 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JNN59_RS12430 2621897..2622181 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
JNN59_RS12435 2622410..2623063 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
JNN59_RS12440 2623056..2623940 - 885 WP_001240581.1 formate dehydrogenase N subunit beta -
JNN59_RS12445 2623953..2627000 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T188371 WP_000605675.1 NZ_CP068817:2621619-2621897 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T188371 NZ_CP090414:c1231716-1231423 [Bifidobacterium longum]
ATGCTCAAACCGGATTACACGGCGGCATTCCAGCGAGATATCAAGAAGCTCAAACGCAAGCACGCTGATTTACGCCCGCT
GAAAGAAGTCATTCGTCTGGTGCTTGAGGACACGGCGGACTCCAAGGAAGCATTGAGGCGTAGGCATCGCGCCCACACAC
TTACCGGTGATCTAAACGGCGTACTGGAATGCCACATCGGCAATGCCGGCGACTGGCTGCTTCTATGGATTCGCGATGAT
GGTACCGCCATGTTCATGCGAACCGGCAGCCATGACGAGCTGTTGGGCAAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT188371 WP_000781370.1 NZ_CP068817:2621897-2622181 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT188371 NZ_CP090414:c1231989-1231720 [Bifidobacterium longum]
GTGAGCATGGCATCCGTCACAGTCCGCGTGGATGCGGACACCAAGCAGAAAGCCGCAAACATAGCCGAGGATCTCGGTCT
GGACTTGTCTTCGGTCACCCGCGCCTTCTACAAGCAGATTGTCCGGGAGAAGCGCATTCCCCTAAACCTTTCCTATACGA
TCGTGCCGCCTGAAACCATGGAGGCCCTTGACGAGGCGAAGAACAGGCTCGCCGACGGCCAACCTCGCTTCAACAGTGCC
GATGAGATGTTCGATTCGTTGGGAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References