Detailed information of TA system TA188353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4950766..4951368
• Replicon: chromosome
• Accession: NZ_CP068816
• Organism: Escherichia coli strain RIVM_C028724

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JNN36_RS23605
• Protein ID: WP_000897305.1
• Coordinates: 4951057..4951368 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JNN36_RS23600
• Protein ID: WP_000356397.1
• Coordinates: 4950766..4951056 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN36_RS23580	4947268..4948170	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JNN36_RS23585	4948167..4948802	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JNN36_RS23590	4948799..4949728	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JNN36_RS23595	4949943..4950161	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
JNN36_RS23600	4950766..4951056	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
JNN36_RS23605	4951057..4951368	-	312	WP_000897305.1	hypothetical protein	Toxin
JNN36_RS23610	4951597..4952505	+	909	WP_001298596.1	alpha/beta hydrolase	-
JNN36_RS23615	4952569..4953510	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JNN36_RS23620	4953555..4953992	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JNN36_RS23625	4953989..4954861	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JNN36_RS23630	4954855..4955454	-	600	WP_001298594.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T188353 WP_000897305.1 NZ_CP068816:c4951368-4951057 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T188353 NZ_CP090402:c4154962-4154612 [Shigella sp. PIB]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT188353 WP_000356397.1 NZ_CP068816:c4951056-4950766 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT188353 NZ_CP090402:c4155207-4154956 [Shigella sp. PIB]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7