Detailed information of TA system TA188347

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4341918..4342330
• Replicon: chromosome
• Accession: NZ_CP068816
• Organism: Escherichia coli strain RIVM_C028724

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: JNN36_RS20800
• Protein ID: WP_000132614.1
• Coordinates: 4341989..4342330 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4341918..4341994 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN36_RS20790	4338593..4340062	+	1470	WP_001540814.1	type I restriction-modification system subunit M	-
JNN36_RS20795	4340062..4341768	+	1707	WP_001100194.1	restriction endonuclease subunit S	-
-	4341918..4341994	-	77	NuclAT_6	-	Antitoxin
-	4341918..4341994	-	77	NuclAT_6	-	Antitoxin
-	4341918..4341994	-	77	NuclAT_6	-	Antitoxin
-	4341918..4341994	-	77	NuclAT_6	-	Antitoxin
-	4341918..4341994	-	77	NuclAT_7	-	Antitoxin
-	4341918..4341994	-	77	NuclAT_7	-	Antitoxin
-	4341918..4341994	-	77	NuclAT_7	-	Antitoxin
-	4341918..4341994	-	77	NuclAT_7	-	Antitoxin
JNN36_RS20800	4341989..4342330	+	342	WP_000132614.1	endoribonuclease SymE	Toxin
JNN36_RS20805	4342377..4344470	-	2094	WP_000648237.1	DUF262 domain-containing protein	-
JNN36_RS20810	4344568..4344648	-	81	WP_020233658.1	hypothetical protein	-
JNN36_RS20815	4344876..4345796	-	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
JNN36_RS20820	4345981..4347261	+	1281	WP_001298033.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	4325797..4344533	18736

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T188347 WP_000132614.1 NZ_CP068816:4341989-4342330 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T188347 NZ_CP090402:2652278-2652385 [Shigella sp. PIB]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT188347 NZ_CP068816:c4341994-4341918 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin