Detailed information of TA system TA188343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3948305..3948999
• Replicon: chromosome
• Accession: NZ_CP068816
• Organism: Escherichia coli strain RIVM_C028724

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: JNN36_RS18910
• Protein ID: WP_001263500.1
• Coordinates: 3948305..3948703 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: JNN36_RS18915
• Protein ID: WP_000554758.1
• Coordinates: 3948706..3948999 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN36_RS18885	3943670..3944128	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
JNN36_RS18890	3944389..3945846	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
JNN36_RS18895	3945903..3946517	-	615	WP_000602124.1	peptide chain release factor H	-
JNN36_RS18900	3946514..3947653	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
JNN36_RS18905	3947843..3948295	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
JNN36_RS18910	3948305..3948703	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JNN36_RS18915	3948706..3948999	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JNN36_RS18920	3949051..3950106	-	1056	WP_001226168.1	DNA polymerase IV	-
JNN36_RS18925	3950177..3950962	-	786	WP_000207578.1	putative lateral flagellar export/assembly protein LafU	-
JNN36_RS18930	3950934..3952646	+	1713	Protein_3714	flagellar biosynthesis protein FlhA	-
JNN36_RS18935	3952674..3952883	+	210	WP_071778446.1	hypothetical protein	-
JNN36_RS18940	3952966..3953463	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3948305..3968659	20354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T188343 WP_001263500.1 NZ_CP068816:c3948703-3948305 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T188343 NZ_CP090402:2041926-2042029 [Shigella sp. PIB]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT188343 WP_000554758.1 NZ_CP068816:c3948999-3948706 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT188343 NZ_CP090402:c2042067-2041799 [Shigella sp. PIB]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCTG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3