Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2577130..2577692 Replicon chromosome
Accession NZ_CP068816
Organism Escherichia coli strain RIVM_C028724

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag JNN36_RS12130 Protein ID WP_000605675.1
Coordinates 2577130..2577408 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JNN36_RS12135 Protein ID WP_000781370.1
Coordinates 2577408..2577692 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN36_RS12105 2572716..2573147 - 432 WP_000152305.1 peroxiredoxin OsmC -
JNN36_RS12110 2573492..2573707 + 216 WP_000495766.1 biofilm-dependent modulation protein -
JNN36_RS12115 2573809..2573946 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
JNN36_RS12120 2574103..2575800 + 1698 WP_000433462.1 malate dehydrogenase -
JNN36_RS12125 2575934..2576944 + 1011 WP_000642380.1 alcohol dehydrogenase AdhP -
JNN36_RS12130 2577130..2577408 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JNN36_RS12135 2577408..2577692 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
JNN36_RS12140 2577921..2578574 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
JNN36_RS12145 2578567..2579451 - 885 WP_001240581.1 formate dehydrogenase N subunit beta -
JNN36_RS12150 2579464..2582511 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T188337 WP_000605675.1 NZ_CP068816:2577130-2577408 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T188337 NZ_CP090402:296215-296466 [Shigella sp. PIB]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT188337 WP_000781370.1 NZ_CP068816:2577408-2577692 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT188337 NZ_CP090402:296487-296846 [Shigella sp. PIB]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCATACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTACTAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References