Detailed information of TA system TA188322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 608578..609377
• Replicon: chromosome
• Accession: NZ_CP068816
• Organism: Escherichia coli strain RIVM_C028724

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: JNN36_RS02940
• Protein ID: WP_000347252.1
• Coordinates: 608578..609042 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: JNN36_RS02945
• Protein ID: WP_001296435.1
• Coordinates: 609042..609377 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN36_RS02910	603579..604013	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
JNN36_RS02915	604031..604909	-	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JNN36_RS02920	604899..605678	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
JNN36_RS02925	605689..606162	-	474	WP_001298322.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JNN36_RS02930	606185..607465	-	1281	WP_000681930.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JNN36_RS02935	607714..608523	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JNN36_RS02940	608578..609042	-	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JNN36_RS02945	609042..609377	-	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JNN36_RS02950	609526..611097	-	1572	WP_001273939.1	galactarate dehydratase	-
JNN36_RS02955	611472..612806	+	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
JNN36_RS02960	612822..613592	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T188322 WP_000347252.1 NZ_CP068816:c609042-608578 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T188322 NZ_CP090400:c846514-845981 [Vibrio cholerae]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT188322 WP_001296435.1 NZ_CP068816:c609377-609042 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT188322 NZ_CP090400:c846780-846511 [Vibrio cholerae]
ATGGCAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTT
AAAAAGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGG
TTAAAGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTT
AAATTTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5